Escherichia coli S fimbriae do not contribute to intestinal colonization or translocation in the gnotobiotic rat. |
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| Authors: | V M Herías A K Robertson T Midtvedt A E Wold |
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| Affiliation: | 1. Department of Clinical Immunology, Göteborg University, Göteborg, Sweden;2. Department of Medical Microbial Ecology, Karolinska Institute, Stockholm, Sweden;1. Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, 5 Yushan Road, Qingdao 266003, China;2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No.1 Wenhai Road, Aoshanwei Town, Jimo, Qingdao 266071, China;1. QOPNA, Mass Spectrometry Center, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal;2. CEB – Centre of Biological Engineering, LIBRO – Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal;3. Anatomia Patológica, Centro Hospitalar Baixo-Vouga, Avenida Artur Ravara, 3814-501 Aveiro, Portugal;4. IBMC – Instituto de Biologia Molecular e Celular, Rua do Campo Alegre 83, Porto, Portugal;5. ICBAS – Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Rua de Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal;6. iBiMED, Institute for Biomedical Research, University of Aveiro, Aveiro, Portugal;2. State Key Laboratory of Applied Microbiology, South China (the Ministry–Province Joint Development), Provincial Key Laboratory of Microbiology Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, 510070, China;3. School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, China;1. Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Kosice, 041 81 Kosice, Slovakia;2. Institute of Neuroimmunology, Slovak Academy of Sciences, 845 10 Bratislava, Slovakia;3. Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, H-1581 Budapest, Hungary;4. Bioinformatics & Medical Informatics Team, Biomedical Research Foundation, Academy of Athens, Athens 11527, Greece;1. Department of Infection Biology, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany;2. Department of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany;3. Department Genetics of Microorganisms, Interfaculty Institute for Genetics and Functional Genomics, Ernst Moritz Arndt University, Greifswald, Germany;4. Faculty of Biology, Friedrich Schiller University, Jena, Germany;1. Whitehead Institute for Biomedical Research, Cambridge, MA;2. Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA;3. Department of Biology, Cambridge, MA;4. Department of Biological Engineering, MIT, Cambridge, MA;5. The Roslin Institute, University of Edinburgh, Edinburgh, United Kingdom;6. Sanford Children''s Health Research Center, La Jolla, CA;7. Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA |
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| Abstract: | Escherichia coli S fimbriae, which bind to sialic acid residues, are a virulence factor for extraintestinal infection, but also promote binding to intestinal epithelial cells. In this study, we investigated whether S fimbriae would enhance intestinal colonization by E. coli or promote translocation to extraintestinal sites. A mixture of two E. coli isogenic strains both expressing type-1 fimbriae but differing in the carriage of S fimbriae (Sfim+ and Sfim-) were given perorally to germfree neonatal, infant or adult rats. The Sfim+ bound better to rat intestinal mucus and epithelial cells. However, both strains colonized equally well in both the small and large intestine and their rate of translocation to the mesenteric lymph nodes was similar. Infant rats had higher E. coli levels in the small intestine than adult rats, but their translocation rates were lower. This was at least partly due to their milk diet, since weaned infant rats had more translocating bacteria than infant rats that continued suckling their mother. The results suggest that S fimbriae, despite binding to intestinal epithelial cells and mucus, do not contribute to either colonization or translocation in the gnotobiotic rat. |
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