检测3种发热性疾病IgM抗体的微孔板蛋白质芯片的研制

〔目的〕针对风疹、麻疹、流行性乙型脑炎3种病毒建立能同时1次检测3种病毒IgM抗体的微孔板蛋白质芯片。〔方法〕将风疹、麻疹和乙脑3种病毒的特异抗原阵列于PVDF微孔板内,建立了在1个反应孔内能同时检测风疹、麻疹、乙脑等3种IgM抗体的蛋白质芯片检测方法。以ELISA实验为对照,通过对239份临床血清样本的分析,系统评价了该芯片系统的灵敏性和特异性。〔结果〕该芯片检测风疹、麻疹、乙型脑炎病毒的灵敏性分别为91.67%、100%、94.12%,特异性分别为99.53%、99.50%、99.10%,与ELISA实验的总符合率依次为98.74%、99.58%、98.74%。〔结论〕本研究建立的微孔板蛋白质芯片检测方法具有准确、简便、快捷、高通量等特点,具有良好的应用前景。

Development of a Micro-well Protein Array for Simultaneous Detection of Three IgM Antibodies in Febrile Diseases

Objective Rubella, measles and epidemic encephalitis B are common human febrile diseases which seriously affect human health and easily cause outbreak and epidemic in population. But the diagnosis methods for pathogenic viruses in clinical laboratories, such as ELISA, are unitary and low efficiency. In this study, we will establish a protein array method based on micro-well plate for simultaneous detection of the three viruses IgM antibodies. Method Three specific antigens of rubella virus, measles virus an...