| 细辛含药血清对SD大鼠DRG神经元IK的影响 |
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| 引用本文： | 杨伟峰,汪琼,周祯祥,陈泽斌,胡平,李晶晶. 细辛含药血清对SD大鼠DRG神经元IK的影响[J]. 湖北中医药大学学报, 2009, 11(5): 6-9 |
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| 作者姓名： | 杨伟峰 汪琼 周祯祥 陈泽斌 胡平 李晶晶 |
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| 作者单位： | 湖北中医学院,湖北,武汉,430061;湖北中医学院,湖北,武汉,430061;湖北中医学院,湖北,武汉,430061;湖北中医学院,湖北,武汉,430061;湖北中医学院,湖北,武汉,430061;湖北中医学院,湖北,武汉,430061 |
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| 基金项目： | 湖北省自然科学基金项目(2005ABA191) |
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| 摘 要： | 目的探讨细辛含药血清对SD大鼠延髓背侧呼吸中枢(DRG)神经元延迟外向整流钾离子通道(IK)的影响。方法将30只SD大鼠随机分为空白血清组和含药血清组各15只,禁食12h后分别灌服细辛散剂药液和等体积的生理盐水,每日1次,连续8d。末次灌胃2h后眼眶采血。室温下静置30min后取上清液,2500r/min离心25min,分离血清,置于56℃水浴30min灭活,过0.22μm微孔滤膜滤过除菌,分装,-20℃条件下保存备用。鼠龄1-3d的SD大鼠,雌雄不限,采用膜片钳WCR,分别记录空白对照组、空白血清组和含药血清组大鼠DRG神经元IK的变化。结果空白血清组与空白对照组相比,无显著性差异(P>0.01),而细辛含药血清与空白对照组、空白血清组相比均有非常显著性差异(P<0.01)。各组大鼠DRG神经元IK的生物特性无明显变化。结论细辛对延髓DRG呼吸神经元延迟外向整流钾离子通道(IK)的激活作用可能是细辛抑制呼吸中枢的离子机制之一。
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| 关 键 词： | 细辛 含药血清 延髓背侧呼吸组神经元 延迟外向整流钾离子通道 实验研究 |
The influence of Asarum medicated serum on the delayed outward potassium current (IK) in DRG neurons in SD rats |
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| YANG Wei-feng,WANG Qiong,ZHOU Zhen-xiang,CHEN Ze-bin,HU Ping,LI Jing-jing. The influence of Asarum medicated serum on the delayed outward potassium current (IK) in DRG neurons in SD rats[J]. Journal of Hubei University of Chinese Medicine, 2009, 11(5): 6-9 |
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| Authors: | YANG Wei-feng WANG Qiong ZHOU Zhen-xiang CHEN Ze-bin HU Ping LI Jing-jing |
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| Affiliation: | Hubei University of Chinese Medicine;Wuhan 430061;Hubei |
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| Abstract: | Objective: To observe the influence of Asarum medicated serum on the delayed outward potassium channel in isolated SD rat's medulla DRG neurons.Methods 30 SD rats were randomized into control serum group(n=15) and medicated serum group(n=15).After 12 hours fasting,rats of different groups were given respectively Asarum powder and normal saline in equal volume via gastric perfusion,once a day for consecutively 8 days.Orbital blood sampling was performed at 2 hours after final gastric perfusion.The supernatan... |
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| Keywords: | Asarum Medicated serum Medullary inspiratory neurons of dorsal respiratory group Delayed outward potassium channel: Experimental research |
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