Comparative cytotoxicity of five current dentin bonding agents: Role of cell cycle deregulation |
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Authors: | Hung-Wei Yeh Mei-Chi Chang Chun-Pin Lin Wan-Yu Tseng Hsiao-Hua Chang Tong-Mei Wang Yi-Jane Chen Chiu-Chun Lin Ting-Ting Yang Li-Deh Lin Jiiang-Huei Jeng |
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Affiliation: | aLaboratory of Pharmacology and Toxicology, Department of Dentistry & School of Dentistry, National Taiwan University Hospital and National Taiwan University Medical College, Taipei, Taiwan;bBiomedical Science Team, Chang Gung Institute of Technology, Taoyuan, Taiwan;cDepartment of Dentistry, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan;dDepartment of Dentistry, Hsin-Chu Municipal Hospital, Hsin-Chu, Taiwan |
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Abstract: | To compare the cytotoxicity of three nano-dentin bonding agents (nano-DBAs) and two non-nano-DBAs using Chinese hamster ovary (CHO-K1) cells. We found that nano fillers were not the major contributing factor in DBA cytotoxicity, as analyzed by colony forming assay and 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Exposure of CHO-K1 cells to all three tested total-etching DBAs led to G0/G1 cell cycle arrest, whereas exposure to higher concentrations of two tested nano-DBAs induced G2/M arrest. All five DBAs further induced apoptosis at the highest concentration, as analyzed by propidium iodide staining flow cytometry. The toxicity of all DBAs (1:4000 v/v or higher) is related to increased reactive oxygen species (ROS) production, as analyzed by single cell DCF fluorescence flow cytometry. These results indicate that clinical application of DBAs may be potentially toxic to dental pulp tissues. Cytotoxicity of DBAs is associated with ROS production, cell cycle deregulation and apoptosis. Presence of methacrylate monomers such as PENTA and UDMA is possibly the major cytotoxic factor for DBAs. Further studies on other toxicological endpoints of nano-DBAs are necessary to highlight their safe use. |
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Keywords: | Apoptosis Cell cycle CHO-K1 cells Cytotoxicity Dentin bonding agents |
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