| HPLC法测定青黛中靛兰和靛玉红的含量的研究 |
| |
| 引用本文： | 戴富宝,乔传卓,李玲.HPLC法测定青黛中靛兰和靛玉红的含量的研究[J].药学学报,1986,21(11):868-871. |
| |
| 作者姓名： | 戴富宝 乔传卓 李玲 |
| |
| 作者单位： | 第二军医大学药学系 上海
(戴富宝，乔传卓)，第二军医大学药学系 上海(李玲) |
| |
| 摘 要： | 青黛为爵床科植物马蓝(Baphicacanthus cusia Bremek.)、十字花科植物菘蓝(Isatis indigotiea Fort.)、豆科植物野青树(Indigofera suffruticosa Mill.)、蓼科植物蓼蓝(Polygonum tinctorium Ait.)的叶或茎叶,经加工制得的干燥粉末或团块。是一种常用中药,
|
| 关 键 词： | 靛蓝 靛玉红 色胺酮 高效液相层析法 |
| 收稿时间： | 1986-01-27 |
DETERMINATION OF INDIGO AND INDIRUBIN IN QINGDAI BY HPLC |
| |
| DAI Fu-Bao,QIAO Chuan-Zhuo and LI Ling.DETERMINATION OF INDIGO AND INDIRUBIN IN QINGDAI BY HPLC[J].Acta Pharmaceutica Sinica,1986,21(11):868-871. |
| |
| Authors: | DAI Fu-Bao QIAO Chuan-Zhuo and LI Ling |
| |
| Abstract: | A HPLC method has been established to analyze indigo, and indirubin in Oingdai under the following conditions. Column: LiChrosorb RP-18 10μm, 2.6×250mm; flow rate: 1.5ml/min; detector: LC-65T (Perkin-Elmer) at 292 nm; sensitivity: 0.02 aufs.Tryptanthrin was used as the internal standard and peak height ratio or peak area ratio was used for quantitative determination This method showed good linearity (r=0.999. n=5) in the range of 2.4～12 μg/ml. The average recovery of indigo is 99.07～103.70% with CV<2% (n=3), and that of indirubin is 99.49～101.23% with CV<2% (n=3).This method is sensitive, rapid, simple and showed good reproducibility. |
| |
| Keywords: | Indigo Indirubin Tryptanthrin HPLC |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《药学学报》浏览原始摘要信息 |
| 点击此处可从《药学学报》下载免费的PDF全文 |
