甲基莲心碱对H202损伤心肌细胞的保护作用

目的：探讨甲基莲心碱（neferine）对氧化损伤的大鼠乳鼠心肌细胞的保护作用。方法：分离培养sD乳鼠心肌细胞，建立心肌细胞过氧化损伤模型，将细胞分为7组，即空白对照组（contr01）、溶剂对照组（DMSO）、氧化损伤组（H202）、氧化损伤加入槲皮素对照组（quercetin＋H202）、氧化损伤加入莲心碱低、中、高浓度组（甲基莲心碱＋H202、甲基莲心碱一M＋H202、甲基莲心碱一H＋H202）。将3001xmol／LH202作用于加入槲皮素及不同浓度甲基莲心碱预培2h的心肌细胞，继续培养24h，以细胞毒四唑盐（MTr）比色试验，乳酸脱氢酶（LDH）、谷胱甘肽过氧化物酶（GSH—px）、丙二醛（MDA）、流式细胞凋亡率、Caspase酶活性为检测指标。结果：甲基莲心碱呈剂量依赖性降低H202对心肌细胞活力的影响，降低MDA、LDH量，增加细胞GSH—px活性，并显著抑制Caspase一3和9活性，减少细胞凋亡数量，各指标差异具有统计学意义（P〈0．01）。结论：甲基莲心碱可保护和修复过氧化氢诱导的心肌细胞的损伤，其作用可能是通过抗氧化、增加细胞GSH—px活性，进而抑制Caspase一3和9活性，减少细胞凋亡。

Protective Effects of Neferine on Cardiomyocyte Ceils Injuries Induced by H202

Objective ：To study the effect of neferine on cardiomyocyte cells injuries induced by H2 02. Methods：Endo- thelial cells were cultured in vitro and divided into seven groups：control group, solvent group, H2 02 group, quercetin plus H202 group, neferine - L, M, H plus H2 02 groups respectively. Cardiomyocyte cells were incubated with 300 .9 mol/L H202 for 24 hours in the absence or presence of various concentrations of neferine and quereetin. Then the apoptotie inde- xes were detected by flow eytometrie. Meanwhile, the amount of Malondialdehyde （ MDA）, the activty of GSH - px and caspase were measured. Results：Incubation of eardiomyoeyte cells with H2 02 for 24h decreased the rate of apoptotie cells, the release of LDH,inhibited the activity of caspase - 3 and 9, and reduced the contents of MDA, accompanied by the de- crease in GSH -px activity. Pretreatment of neferine with 1 ～ 30μmol decreased these H202 -induced changes in a con- centration - dependent manner（ P 〈 0.01 ） . Conclusion ： Neferine posses a protective effect against eardiomyocyte cells in- juries induced by H2 02, which is probably via anti - oxidation, increasing the activity of GSH - px, reducing caspase - 3 and 9 activity and inhibiting the apoptosis of endothelial cells.