| PHA,抗CD3单抗,IL—2诱导脾细胞增殖及其抗肿瘤效应的实验研究 |
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| 引用本文： | 王晓林 朱慧芬. PHA,抗CD3单抗,IL—2诱导脾细胞增殖及其抗肿瘤效应的实验研究[J]. 中国肿瘤临床, 1994, 21(10): 725-727 |
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| 作者姓名： | 王晓林 朱慧芬 |
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| 作者单位： | 同济医科大学实验医学研究中心!武汉430030 |
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| 基金项目： | 卫生部青年基金资助课题(1993) |
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| 摘 要： | 本文应用PHA、抗CD_3单抗、IL-2体外诱导正常人脾细胞增殖,并探讨了在不同条件的诱导下脾细胞体外增殖能力和抗肿瘤效应,以及细胞表面标志的表达特征.结果显示,抗CD_3单抗、PHA不仅可增强IL-2诱导脾细胞的增殖作用,且两者合用有协同增强效应.PHA、抗CD_3单抗还可增强体外扩增细胞CD_4和Tas受体的表达.LAK细胞抗肿瘤活性测定结果提示,PHA有直接和间接增强LAK细胞抗肿瘤效应的作用,抗CD_3单抗则可通过增加细胞增殖作用,间接地增强LAK细胞的抗肿瘤活性.
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| 关 键 词： | 抗CD_3单抗 PHA rhIL-2 LAK细胞 |
The Proliferation and Anti-tumor Effect of Splenocytes Induced by PHA,Anti-CD3 McAb,and IL-2 |
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| Wang Xiao-Lin et al. The Proliferation and Anti-tumor Effect of Splenocytes Induced by PHA,Anti-CD3 McAb,and IL-2[J]. Chinese Journal of Clinical Oncology, 1994, 21(10): 725-727 |
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| Authors: | Wang Xiao-Lin et al |
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| Affiliation: | Wang Xiao-Lin et alDepartment of Immunology,Experimental Research Center of Medicine,Tongji Medical University,Wuhan |
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| Abstract: | The proliferation of splenocyte derived from healthy adults was induced by PHA, anti-CD3 MCAb,and IL-2. The proliferative capability and anti-tumor activity as well as phenotypes of splenocytes cultured in different systems were studied. The results showed that anti-CD3 McAb and PHA enhanced the proliferation of splenocytes induced by IL-2, not only separately but also syntonically. The expressions of CD, and Tac on cell surface increased after the splenocytes being induced by anti-CD3 McAb and PHA. The results of anti-tumor activity of LAK cells suggested that PHA had capability to promote anti-tumor activity of LAK cells both directly and indirectly and that anti-CD3 McAb promoted anti-tumor activity of LAK cells by an indirect way of enhancing splenocytes proliferation. |
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| Keywords: | Anti-CD3 McAb PHA A%#AAIL-2 2%#22LAK cell |
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