十味接骨药对家兔骨折骨细胞生长机理的实验研究

目的研究十味接骨药对家兔骨折骨细胞生长修复过程中骨痂中TGF-β1,羟脯氨酸、钙、磷 血清中碱性磷酸酶、钙、磷的变化和作用机理。方法实验用健康日本大白兔81只5～6月龄造成双侧前肢0.7cm横断性骨缺损,保留尺骨完整,并分成三组,对照组和实验组,空白组不用药,实验组给十味接骨药,对照组给骨折挫伤散。2周、5周、8周分别取材,观察十味接骨药对骨折修复的影响。结果实验组骨痂中TGF-β1、HRP先升高后递减 血中骨痂中Ca、P峰值提前出现,血中ALP随骨生长逐渐升高。与对照组相比较统计学＊P〈0.01,＊＊P〈0.001。结论十味接骨药对骨折的愈合修复有显著促进作用。

Therapeutic Effect of SHI-WEI-JIE-GU-YAO on Experimental Fracture and Its Mechanism

Objective The article compares the bias assessments of turbidimetric immunoassay method and dry-slide bromocresol green method in ALB detection,and discusses whether the bias of the two detection results are within the range permitted,so as to ensure the comparability of the results.Method According to NCCLS standardization document EP9-A2 1],firstly 8 clinical samples are daily taken and tested duplicates under each method,altogether five days.Next the outlier points are excluded and correlation coefficients and expected deviations are calculated.The value is restricted by 1/2 of the error that external quality assessment allows（ALB＋10%）by CLIA＇88 as the method comparison result system error,and then it is bias assessed.Results The results of comparison between dry-slide bromocresol green method and turbidimetric immunoassay method are as follows：r2=0.934,when medical decisions are 20 g/L,35 g/L and 52 g/L,the expected relative deviations of dry-slide bromocresol green method are 10.4%,6.8%,3.2% respectively.When Xc=20 g/L and Xc=35 g/L,there are significant differences between the results of two methods.Conclusion Turbidimetric immunoassay is regarded as the methodology in comparison.Dry-slide bromocresol green method,however,proves positive deviations in the results of ALB determination.Its value of bias increases with the decline of ALB concentration,and it is more apparent when the concentration turns low.These two methods have poor correlation.Therefore,when different analysis methods are utilized in the detection of the same object,it is suggested different methodological comparisons and bias assessments be used in each method to make clear the disparities among them and then establish each reference range,especially the concentration reference values of different methods in different medical decisions,to insure the comparability of the detection results.