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N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸对血清诱导的心脏成纤维细胞MMP-2、MMP-9活性表达的调节作用
引用本文:马文东,张丽娟,罗玲,裴新,户万秘,杨方. N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸对血清诱导的心脏成纤维细胞MMP-2、MMP-9活性表达的调节作用[J]. 华北煤炭医学院学报, 2008, 10(2): 147-148
作者姓名:马文东  张丽娟  罗玲  裴新  户万秘  杨方
作者单位:华北煤炭医学院实验中心,河北唐山,063000
基金项目:河北省科技厅博士基金(04547002D-5)资助项目,河北省教育厅基金(2003112)资助项目,唐山市新医药基础研究重点实验室项目(04362001B)
摘    要:①目的探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)对不同浓度血清(FBS)诱导的大鼠心脏成纤维细胞MMP-2、MMP-9酶活性表达的调节作用。②方法选用新生大鼠心脏成纤维细胞;采用明胶酶谱法检测心脏成纤维细胞MMP-2、MMP-9酶活性的表达。③结果在0.4%、2%和10%几种血清浓度的条件下,随着血清浓度的增加,MMP-2、MMP-9酶活性表达增强。AcS-DKP能够进一步增加由10%血清诱导的心脏成纤雏细胞MMP-2、MMP-9酶活性的表达。④结论AcSDKP上调了由血清介导的心成纤维细胞MMP-2,MMP-9酶活性表达,这可能与AcSDKP抗心脏纤维化的作用相关。

关 键 词:N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸  血清  心脏成纤维细胞  基质金属蛋白酶
文章编号:1008-6633(2008)02-147-02
收稿时间:2008-01-24
修稿时间:2008-01-24

Effect of AcSDKP on activities of MMP - 2 and MMP - 9 in cardiac fibroblast induced by FBS
Ma Wendong, Zhang Lijuan, Luo Ling ,et al. Effect of AcSDKP on activities of MMP - 2 and MMP - 9 in cardiac fibroblast induced by FBS[J]. Journal of North China Coal Medical College, 2008, 10(2): 147-148
Authors:Ma Wendong   Zhang Lijuan   Luo Ling   et al
Affiliation:Ma Wendong, Zhang Lijuan, Luo Ling ,et al( Experimental and Research Center, North China Coal Medical University, Tangshan 063000, China)
Abstract:Objective To investigate the regulation effects of AcSDKP on activity expressions of MMP-2 and MMP-9 in cultured rat cardiac fibroblasts mediated by different concentration fetal bovine serum(FBS).Methods Neonatal rat cardiac fibroblasts were isolated.The MMP-2 and MMP-9 activity expressions were measured with zymography assays.Results MMP-2 and MMP-9 activity expressions were increased as FBS increase at the 0.4%,2% and 10% concentrations.MMP-2 and MMP-9 activity expression in cardiac fibroblasts induced by 10% FBS were further increased by AcSDKP.Conclusion MMP-2 and MMP-9 activity expressions in cardiac fibroblasts mediated by FBS were up-regulated by AcSDKP,which was possible related with the effect of AcSDKP anti-fibrosis.
Keywords:N-acetyl-seryl-aspartyl-lysyl-proline.Fetal bovine serums.Cardiac fibroblasts.Matrix metalloproteinases
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