| N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸对血清诱导的心脏成纤维细胞MMP-2、MMP-9活性表达的调节作用 |
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| 引用本文： | 马文东,张丽娟,罗玲,裴新,户万秘,杨方.N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸对血清诱导的心脏成纤维细胞MMP-2、MMP-9活性表达的调节作用[J].华北煤炭医学院学报,2008,10(2):147-148. |
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| 作者姓名： | 马文东 张丽娟 罗玲 裴新 户万秘 杨方 |
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| 作者单位： | 华北煤炭医学院实验中心,河北唐山,063000 |
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| 基金项目： | 河北省科技厅博士基金(04547002D-5)资助项目，河北省教育厅基金(2003112)资助项目，唐山市新医药基础研究重点实验室项目(04362001B) |
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| 摘 要： | ①目的探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸（AcSDKP）对不同浓度血清（FBS）诱导的大鼠心脏成纤维细胞MMP-2、MMP-9酶活性表达的调节作用。②方法选用新生大鼠心脏成纤维细胞；采用明胶酶谱法检测心脏成纤维细胞MMP-2、MMP-9酶活性的表达。③结果在0．4％、2％和10％几种血清浓度的条件下，随着血清浓度的增加，MMP-2、MMP-9酶活性表达增强。AcS-DKP能够进一步增加由10％血清诱导的心脏成纤雏细胞MMP-2、MMP-9酶活性的表达。④结论AcSDKP上调了由血清介导的心成纤维细胞MMP-2，MMP-9酶活性表达，这可能与AcSDKP抗心脏纤维化的作用相关。
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| 关 键 词： | N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸 血清 心脏成纤维细胞 基质金属蛋白酶 |
| 文章编号： | 1008-6633（2008）02-147-02 |
| 收稿时间： | 2008-01-24 |
| 修稿时间： | 2008年1月24日 |
Effect of AcSDKP on activities of MMP - 2 and MMP - 9 in cardiac fibroblast induced by FBS |
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| Ma Wendong, Zhang Lijuan, Luo Ling ,et al.Effect of AcSDKP on activities of MMP - 2 and MMP - 9 in cardiac fibroblast induced by FBS[J].Journal of North China Coal Medical College,2008,10(2):147-148. |
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| Authors: | Ma Wendong Zhang Lijuan Luo Ling |
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| Institution: | Ma Wendong, Zhang Lijuan, Luo Ling ,et al( Experimental and Research Center, North China Coal Medical University, Tangshan 063000, China) |
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| Abstract: | Objective To investigate the regulation effects of AcSDKP on activity expressions of MMP-2 and MMP-9 in cultured rat cardiac fibroblasts mediated by different concentration fetal bovine serum(FBS).Methods Neonatal rat cardiac fibroblasts were isolated.The MMP-2 and MMP-9 activity expressions were measured with zymography assays.Results MMP-2 and MMP-9 activity expressions were increased as FBS increase at the 0.4%,2% and 10% concentrations.MMP-2 and MMP-9 activity expression in cardiac fibroblasts induced by 10% FBS were further increased by AcSDKP.Conclusion MMP-2 and MMP-9 activity expressions in cardiac fibroblasts mediated by FBS were up-regulated by AcSDKP,which was possible related with the effect of AcSDKP anti-fibrosis. |
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| Keywords: | N-acetyl-seryl-aspartyl-lysyl-proline Fetal bovine serums Cardiac fibroblasts Matrix metalloproteinases |
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