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Reprogramming of adult human neural stem cells into induced pluripotent stem cells |
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| Authors: | XIE Li-qian SUN Hua-ping WANG Tian TANG Hai-liang WANG Pu ZHU Jian-hong YAO Zheng-wei |
| Affiliation: | 1. Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China 2. Department of Radiology ,Huashan Hospital, Fudan University, Shanghai 200040, China 3. National Key Laboratory of Medical Neurobiology, Institute of Brain Science, Shanghai Medical College, Fudan University,Shanghai 200032, China 4. Department of Neurosurgery , Huashan Hospital, Fudan University, Shanghai 200040, China 5. Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China ;National Key Laboratory of Medical Neurobiology, Institute of Brain Science, Shanghai Medical College, Fudan University,Shanghai 200032, China 6. Department of Radiology,Huashan Hospital, Fudan University, Shanghai 200040, China |
| Abstract: | Background Since an effective method for generating induced pluripotent stem cells (iPSCs) from human neural stem cells (hNSCs) can offer us a promising tool for studying brain diseases,here we reported direct reprogramming of adult hNSCs into iPSCs by retroviral transduction of four defined factors.Methods NSCs were successfully isolated and cultured from the hippocampus tissue of epilepsy patients.When combined with four factors (OCT3/4,SOX2,KLF4,and c-MYC),iPSCs colonies were successfully obtained.Results Morphological characterization and specific genetic expression confirmed that these hNSCs-derived iPSCs showed embryonic stem cells-like properties,which include the ability to differentiate into all three germ layers both in vitro and in vivo.Conclusion Our method would be useful for generating human iPSCs from NSCs and provide an important tool for studying neurological diseases. |
| Keywords: | induced pluripotent stem cells ＂ human neural stem cells ＂ reprogramming |
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