日本血吸虫重组抗原rSjGST-32诱导小鼠保护性免疫效果的比较

目的　探索日本血吸虫重组抗原rSjGST-32适合的免疫剂量。　方法　以50、100、200μg3个不同剂量rSjGST-32加佐剂皂角甙A(QuilA)50μg免疫BALB/c小鼠,同时设QuilA和PBS对照组。ELISA法检测抗体水平。末次免疫4wk后攻击感染,用减虫率及减卵率表示保护力。　结果　与PBS对照组比较,50、100、200μgrSjGST-32组的减虫率分别为38.1%,47.8%和48.8%;每克肝卵(LEPG)减少率分别为39.1%,53.5%和53.6%;每条雌虫每克肝所含虫卵数(LEPF)减少率分别为22.5%,22.8%和21.8%;抗体滴度分别达1∶51200,1∶102400和1∶102400。　结论　重组抗原rSjGST-32加QuilA佐剂免疫小鼠,以100μgrSjGST-32剂量较为适合。

Protective Effect Against Schistosoma japonicum of Recombinant Fusion Protein SjGST-32 in Mice

OBJECTIVE: To explore the optimal immune doses of recombinant antigen rSjGST-32, with QuilA as adjuvant. METHODS: BALB/c mice were immunized with doses of 50, 100 and 200 micrograms rSjGST-32/mouse plus 50 micrograms QuilA adjuvant. QuilA and PBS were used as controls. Levels of specific antibodies were detected by ELISA. The mice were challenged 4 weeks after last vaccination. Worms and eggs collected from the livers of mice were counted 45 days after challenge. RESULTS: As compared with the control groups, the worm reduction rate in the 50, 100 and 200 micrograms experiment groups was 38.1%, 47.8% and 48.8%, respectively. The reduction rate of liver eggs per gram (LEPG) was 39.1%, 53.5% and 53.6%, respectively, and the reduction rate of the liver eggs per female (LEPF) was 22.5%, 22.8% and 21.8%, respectively. The specific antibody titers in sera reached 1:51,200, 1:102,400 and 1:102,400, respectively before challenge. CONCLUSION: The results show that for vaccinating BALB/c mice, the optimal antigen dose is 100 micrograms of recombinant rSjGST-32 plus QuilA adjuvant.