| 人参皂苷Rh2对人食管癌Eca-109细胞TFPI-2表达的影响 |
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| 引用本文： | 岳峰,周进朝. 人参皂苷Rh2对人食管癌Eca-109细胞TFPI-2表达的影响[J]. 现代中西医结合杂志, 2012, 21(21): 2304-2306 |
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| 作者姓名： | 岳峰 周进朝 |
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| 作者单位： | 1. 河北省邯郸市中心医院,河北,邯郸,056002
2. 河北省永年县第二医院,河北,永年,057150 |
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| 基金项目： | 河北省科技研究与发展计划项目 |
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| 摘 要： | 目的探讨人参皂苷Rh2对人食管癌Eca-109细胞组织因子途径抑制物2(TFPI-2)表达的影响。方法按人参皂苷Rh2不同浓度设置3个实验组(10,40,80 mg/L)和1个对照组,分别采用RT-PCR法检测细胞TFPI-2 mRNA表达水平、Western blot法检测TFPI-2蛋白表达水平以及Transwell小室法检测细胞的侵袭能力等。结果 10,40,80 mg/L人参皂苷Rh2均可抑制人食管癌细胞Eca-109的侵袭力并能上调TFPI-2 mRNA及蛋白的表达,且40,80 mg/L与对照组比较有显著性差异(P均<0.05)。结论人参皂苷Rh2具有上调人食管癌Eca-109细胞TFPI-2基因表达和抑制其侵袭力的作用。
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| 关 键 词： | 人参皂苷Rh2 食管癌 Eca-109细胞 组织因子途径抑制物2 |
Influence of GS-Rh2 on expression of TFPI-2 of human esophageal cancer Eca-109 cells |
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| Yue Feng , Zhou Jinchao. Influence of GS-Rh2 on expression of TFPI-2 of human esophageal cancer Eca-109 cells[J]. Modern Journal of Integrated Chinese Traditional and Western Medicine, 2012, 21(21): 2304-2306 |
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| Authors: | Yue Feng Zhou Jinchao |
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| Affiliation: | 1.Central Hospital of Handan,Handan 056002,Hebei,China;2.The Second Hospital of Yongnian County,Yongnian 057150,Hebei,China) |
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| Abstract: | Objective It is to approach the influence of GS-Rh2 on expression of TFPI-2 of human esophageal cancer Eca-109 cells.Methods After treatment with different concentration of GS-Rh2 for 48h,the expression of TFPI-2 mRNA of Eca-109 cells was measured with RT-PCR,the expression of TFPI-2 protein of Eca-109 cells was measured with western blot and the invasion of Eca-109 cells was analyzed by Transwell invasion assay.Results Treatment with GS-Rh2 at concentration of 40 and 80 mg/L significantly inhibited the invasion and increased the TFPI-2 mRNA and protein of Eca-109 cells than control group(P<0.05),GS-Rh2 of 10 mg/L was so,but no significant than control group(P>0.05).Conclusion GS-Rh2 can increase the expression of TFPI-2 mRNA and protein of Eca-109 cells,and inhibit the invasion of its. |
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| Keywords: | GS-Rh2 esophageal cancer Eca-109 cell TFPI-2 |
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