ALDH1、Twist在胃腺癌组织中的表达及临床意义

目的 分析乙醛脱氢酶1（ALDH1）、Twist在胃腺癌（GAC）组织中的表达及意义。方法 回顾性分析86例GAC患者临床病理资料及随访结果。对86例GAC标本及30例正常对照组织（随机选取其中30例GAC患者术中不含癌组织的切缘组织）采用原位杂交实验检测ALDH1、Twist mRNA表达，采用免疫组化染色法检测ALDH1、Twist、上皮间质转化相关因子E-cadherin、N-cadherin蛋白的表达，分析其与患者临床病理参数、术后生存时间的关系，探讨ALDH1、Twist的表达与上皮间质转化的关系。结果 GAC组织中ALDH1、Twist mRNA和蛋白的表达阳性率分别为57.0%、53.5%和60.5%、58.1%，与对照组之间差异有统计学意义（P<0.05）。ALDH1蛋白的表达与肿瘤的临床TNM分期和淋巴结转移有关（P<0.05），Twist蛋白的表达与肿瘤远处转移、淋巴结转移及浸润深度有关（P<0.05）。ALDH1、Twist的表达与E-cadherin的低表达和N-cadherin的高表达存在相关性。单因素分析表明TNM分期、淋巴结转移、远处转移、肿瘤大小、ALDH1和Twist的表达与GAC患者术后5年生存率相关（P均<0.05）。结论 GAC组织中ALDH1、Twist 的检测对预测肿瘤侵袭转移、判断预后具有一定价值。

Expression and Clinical Significance of ALDH1 and Twist in Gastric Adenocarcinoma

Objective To investigate the expressions and clinical significances of aldehyde dehydrogenase 1（ALDH1） and Twist in gastric adenocarcinoma. Methods A total of 86 cases of gastric adenocarcinoma was included in this study. GAC specimens and normal gastric tissues were obtained from the patients. in situ hybridization method was used to detect the expression of ALDH1, Twist mRNA． Immuohistochemistry was used to detect the expression of ALDH1, Twist, E-cadherin and N-cadherin. The relationship of ALDH1 and Twist with epithelial-mesenchymal transition phenomenon was studied. Survival analysis was carried to demonstrate the relationships of clinical pathological parameters with 5-year survival rate. Results The positive rates of ALDH1Twist mRNA in GAC tissues were 57.0% and 53.5%, while the positive rates of proteins were 60.5% and 58.1%, which were significantly higher than those in normal controls （P<0.05）. The expression of ALDH1 protein was significantly correlated with TNM stage and lymph node metastasis （P<0.05）.The expression of Twist protein was significantly correlated with distant metastasis, lymph node metastasis and invasion depth (P<0.05). The expressions of ALDH1 and Twist were significantly correlated with low expression of E-cadherin and high expression of N-cadherin（P<0.05）. Univartate analysis showed TNM stage, lymph node metastasis, distant metastasis and tumor size, the expression of ALDH1 and Twist related to 5-year survival rate (P<0.05).Conclusion The enhanced expression of ALDH1 and Twist in gastric adenocarcinoma may play a role in GAC invasion, metastasis and prognosis．