中叶素1-53对大鼠动脉血压的影响及其作用机制

目的研究降钙素/降钙素基因相关肽家族一员的中叶素（IMD）对大鼠动脉血压的影响及其可能机制.方法动脉插管测定大鼠动脉血压,以Greiss法测定孵育血管组织亚硝酸盐含量,用放射方法检测NOS活性及L-Arg摄入.结果IMD具有快速明显的降血压作用,其降压作用较等剂量肾上腺髓质素（ADM）更强.同时IMD可呈浓度依赖性增加血管组织NO含量,eNOS活性及L-Arg摄入,与对照组相比10-7mol/L IMD使上述指标分别增加59%,356%和108%.结论IMD具有强的降低大鼠动脉血压作用,该作用在一定程度上由IMD刺激血管组织NO的生成介导.

Effects of Intermedin1-53 on Rat Arterial Pressure and its Mechanism

Objective The present study was designed to determine the effects of IMD1-53, a member of Calcitonin-CGRP family, on arterial pressure in rats and its possible mechanism. methods Rat arterial pressure was measured after i. v. administration of IMD1-53 3.0 nmol/kg for 45 min. Nitrite concentrations were measured by Greiss method and NOS activation and L-Arg uptake were assayed by radio assay. Results IMD1-53 reduced arterial pressure which was more prominent than that by equipment adrenomedullin. Incubated vascular tissues with IMD1-53 resulted in a concentration-dependent increase in the nitrite content, eNOS activation and L-Arg uptake. As compared with controls, 10~(-7)mol/L IMD 1-53 increased nitrite(+59%), eNOS activation(+356%), and L-Arg uptake(+108%). Conclusion This results suggest that IMD1-53 had substantial hypotensive effect on rat which may be mediated by NO/NOS pathway.