清心开窍方对AD小鼠学习记忆能力及海马CA1区神经细胞形态的影响

目的:探讨清心开窍方对AD模型小鼠学习记忆能力及海马CA1区神经细胞形态的影响。方法:将150只小鼠随机分为5组,即空白组、模型组、清心开窍方高、低剂量组(14.82,7.41 g.kg^-1)、脑复康组(0.42 g.kg^-1)。除空白组外,其余各组均每日灌服三氯化铝(AlCl₃)(200 mg.kg^-1)造模,用药各组自造模第7天起,每日上午给予AlCl₃灌胃,下午给予相应的药物,每日1次,连续8周,9周后评定疗效,测小鼠学习记忆功能和脑内一氧化氮(NO)、一氧化氮合酶(NOS)及乙酰胆碱脂酶(AchE),及对海马CA1区神经细胞形态学观察。结果:中药各组能明显改善AD小鼠学习记忆能力,与对照组比较有显著性差异(P<0.05,P<0.01);清心开窍方各组能明显减少海马神经细胞的破坏,降低AD小鼠脑组织中NO,NOS含量及AchE活性,与模型组比较有统计学意义(P<0.05,P<0.01)。结论:清心开窍方能改善AD小鼠学习记忆的能力,对海马神经细胞具有保护作用。

Effect of Qingxin Kaiqiao fang on learning and memory ability and morphology of hippocampal nerve cells in AD mice

Objective:To study the effect of the Qingxin Kaiqiao fang on learning and memory ability and shape hippocampal nerve cells in Alzheimer disease(AD).Method:One handred and fifty mice were divid into five groups:blank group,model group,two groups of treatment by Qingxin Kaiqiao fang(14.82,7.41 g·kg^-1),piracetan comparison group(0.42 g·kg^-1).The model group was orally given AlCl3(200 mg·kg^-1) every day.For Qingxin Kaiqiaofang and piracetan groups,AlCl₃ treatment was given for 6 days at the beginning,followed by giving orally AlCl₃ in the morning and drug in the afternoon for 8 weeks.Then,learning and memory aility,the contents of nitric oxide(NO),NO synthase(NOS) and acetylcholinesterase(AchE) in brain,and morphology of hippocampal nerve cells were investigated.Result:Learning and memory ability of Qingxin Kaiqiaofang groups was improved,compared with comparison group;the difference was significant(P<0.05,P<0.01).The drug could prevent hippocampal nerve cells from damaged obviously.The contents of NO,NOS and AchE in mice of treatment groups were lower than those of comparison group;the difference was significant(P<0.05,P<0.01).Conclusion:Qingxin Kaiqiaofang can improve learning and memory ability of AD mice,prote chippocampal nerve cells,and treat Alzheimer disease.