消癌平注射液对白血病细胞NB4作用的初步研究

目的研究消癌平注射液对白血病细胞株NB4的效应并初步探讨其机制。方法采用台盼兰染色法检测消癌平注射液对白血病细胞株NB4细胞增殖和活力的影响。流式细胞术分析消癌平注射液对NB4细胞周期和细胞表面分化抗原CD11b表达的影响。NBT还原实验检测对NB4细胞分化的影响。免疫印迹方法检测对细胞周期和凋亡相关蛋白的影响。结果消癌平注射液能以时间和剂量依赖方式抑制白血病细胞NB4增殖。诱导NB4细胞发生G0/G1期阻滞,增加P21蛋白质的水平,但不改变P27的表达。对细胞凋亡相关蛋白质的PARP水平没有影响。消癌平注射液能增加NB4细胞表面抗原CD11b的表达,增加NBT阳性细胞数,诱导NB4细胞分化。结论消癌平注射液能通过诱导细胞周期阻滞和细胞分化抑制NB4细胞增殖。

Preliminary study on effects of Xiaoaiping injection on NB4 cells

Purpose To study the effect of Xiaoaiping injection(XAP) on NB4 leukemia cells and its mechanisms.Methods After being treated with XAP,viability of NB4 leukemia cells as determined by trypan blue assay.Cell cycle was assessed by FACS.Cell differentiation as determined by cell morphology,cell surface differentiation antigen and NBT reduction test.Cell cycle and apoptosis associated proteins were measured by western blot.Results XAP inhibits the growth of leukemia cells in a dose and time dependent manner.XAP induce G0/G1 phase arrest of NB4 cells,accompanied by P21 up-regulation.XAP did not change the protein level of PARP and P27.XAP could increase the expression of CD11b and percent of NBT positive cells,induce differentiation of NB4 cells.Conclusion XAP could inhibit the proliferation of NB4 cells through inducing cell cycle arrest and cell differentiation.