| 干细胞标记物CK19、Notch3、CD133、P75NTR、STRO-1及ABCG2在肺鳞癌中的表达及意义 |
| |
| 引用本文： | 林旭勇,刘树立,刘楠,杨小湜,徐洪涛,王恩华. 干细胞标记物CK19、Notch3、CD133、P75NTR、STRO-1及ABCG2在肺鳞癌中的表达及意义[J]. 中国肺癌杂志, 2009, 12(4). DOI: 10.3779/j.issn.1009-3419.2009.04.11 |
| |
| 作者姓名： | 林旭勇 刘树立 刘楠 杨小湜 徐洪涛 王恩华 |
| |
| 作者单位： | 中国医科大学基础医学院病理学教研室,中国医科大学附属第一医院病理科,沈阳,110001 |
| |
| 摘 要： | 背景与目的 已有研究表明在肿瘤中存在着能够自我更新与多向分化的肿瘤干细胞,它们与正常人体内的干细胞有着相似的特征.本研究选取了被认为可作为干细胞标记物的部分抗体,以观察它们在肺鳞癌中的表达及意义.方法 采用S-P免疫组织化学方法,对54例手术切除肺鳞癌标本进行了CK19、Notch3、CD133、P75NTR、STRO-1及ABCG2的检测,另外选取了10例正常肺组织标本进行对照.结果 54例肺鳞癌标本中,除发现STRO-1及P75NTR在所有病例均为阴性表达外,其余标记均存在不同程度的阳性表达,但是阳性细胞的分布无规律性.其中,CK19阳性率为66.67%(36/54),Notch3为87.04%(47/54),CD133为50%(27/54),ABCG2为61.11%(33/54),并且Notch3、CD133和ABCG2在中低分化肺鳞癌中的表达高于高分化鳞癌(P＜0.05),而CK19、CD133和ABCG2在有淋巴结转移的肺鳞癌中的表达高于无淋巴结转移(P＜0.05),在连续切片中4种标记同时阳性的细胞数均少于总细胞数目的2%,3种及4种抗体共同阳性细胞所占百分比与鳞癌的分化程度和是否有淋巴结转移无统计学相关.结论 肺鳞癌中存在着某些干细胞标记物的表达,其表达程度与肺鳞癌的分化程度及淋巴结转移相关,但是阳性细胞在组织学的分布上无规律性,同时表达多种干细胞标记的肿瘤细胞数少于2%,可能为肿瘤干细胞.
|
| 关 键 词： | 肺肿瘤 干细胞标记物 免疫组织化学 |
Expression and Significance of Stem Cell Markers CK19,Notch3,CD133,P75NTR,STRO-1 and ABCG2 in Pulmonary Squamous Carcinomas |
| |
| Xuyong LIN,Shuli LIU,Nan LIU,Xiaoshi YANG,Hongtao XU,Enhua WANG. Expression and Significance of Stem Cell Markers CK19,Notch3,CD133,P75NTR,STRO-1 and ABCG2 in Pulmonary Squamous Carcinomas[J]. Chinese journal of lung cancer, 2009, 12(4). DOI: 10.3779/j.issn.1009-3419.2009.04.11 |
| |
| Authors: | Xuyong LIN Shuli LIU Nan LIU Xiaoshi YANG Hongtao XU Enhua WANG |
| |
| Affiliation: | Xuyong LIN,Shuli LIU,Nan LIU,Xiaoshi YANG,Hongtao XU,Enhua WANG Department of Pathology,College of Basic Medical Sciences , the First Affiliated Hospital,China Medical University,Shenyang 110001,China |
| |
| Abstract: | Background and objective Increasing reports showed that some tumor stem cells were self-renewal and multi-lineage differentiated in tumors,similar to the normal stem cells in human body.The aim of this study is to observe the expression of stem cell markers in lung squamous carcinoma tissues.Methods Fifty-four lung cancer specimens from surgery were analyzed for CK19,Notch3,CD133,P75NTR,STRO-1 and ABCG2 expression by using S-P immunohistochemistry.In addition,ten normal lung tissue samples were included as ... |
| |
| Keywords: | Lung neoplasms Stem cell marker Immunohistochemistry |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
