福莫司汀对人脑胶质瘤细胞株U251细胞周期的影响

目的:观察福莫司汀(Fotemustine)对人脑胶质瘤细胞株U251细胞增殖的影响。方法:通过体外培养的人脑胶质瘤细胞株U251,用流式细胞仪(flow cytometry,FCM)检测福莫司汀诱导胶质瘤细胞凋亡及其增殖周期的分布;与对照组比较,并进行统计学处理。结果:Fotemustine诱导后,细胞凋亡率明显高于对照组(P<0.05);诱导后细胞,G1期数量减少,而S期和G2期数量增多,与对照组比较差异有显著性(P<0.05)。结论:Fotemustine可诱导人脑胶质瘤细胞株U251的凋亡;诱导细胞增殖周期分布的变化,有助于S期、G2期的化疗药物作用的发挥。

Effect of fotemustine on the cell cycle of human glioma cell line U251

Objective:To investigate the effect of fotemustine on the cell proliferation rates of human glioma cell line U251. Methods:Flow cytometry (FCM) was used to examine the apoptosis and the cycle of cell proliferations, induced by fotemustine, in cultured U251 glioma cells. The results were compared with the control group with statistical analysis. Results:The apoptosis rate of the cell was significantly higher than that of the control group when induced by Fotemustine (P< 0.05 ). After induction, the number of the cells in G1 stage decreased but increased in S stage and G2 stage with marked difference in comparison with the control group (P<0.05 ).Conclusion:Fotemustine can induce the apoptosis of glioma cells U251.The changes in the cell proliferation rates under the induction may improve the curative effects of chemotherapy drugs in S stage and G2 stage.