| 高效液相色谱法测定续断饮片中马钱苷的含量 |
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| 引用本文： | 曹桂侠,汪秀月,焦坤. 高效液相色谱法测定续断饮片中马钱苷的含量[J]. 安徽医药, 2013, 17(5): 768-769 |
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| 作者姓名： | 曹桂侠 汪秀月 焦坤 |
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| 作者单位： | 安徽省阜阳市第四人民医院,安徽,阜阳,236000;安徽省阜阳市食品药品检验所,安徽,阜阳,236015 |
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| 摘 要： | 目的建立中药饮片续断中马钱苷的含量测定方法。方法液相色谱法。色谱柱:InertsilRODS(4.6 mm×150 mm,5μm);流动相:乙腈-0.1%磷酸溶液(10∶90);流速1.0 mL·min-1;柱温:35℃;检测波长:240 nm。结果马钱苷在0.421 2～4.212μg范围内呈线性关系(r=1.000 0,n=6),平均加样回收率为98.9%,RSD为1.6%(n=6)。结论该方法操作简便,结果可靠,可用于中药饮片续断中马钱苷的含量测定。
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| 关 键 词： | 高效液相色谱法 续断 马钱苷 |
Determination of loganin in Radix Dipsaci decocting pieces by HPLC |
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| CAO Gui-xia,WANG Xiu-yue,JIAO Kun. Determination of loganin in Radix Dipsaci decocting pieces by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2013, 17(5): 768-769 |
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| Authors: | CAO Gui-xia WANG Xiu-yue JIAO Kun |
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| Affiliation: | 2 ( 1. Fuyang Fourth People＇s Hospital,Fuyang,Anhui 236000;2. Fufang Institute for Food and Drug,Fuyang,Anhui 236015 ,China) |
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| Abstract: | Objective To establish a method for the determination of loganin in Radix Dipsaci decocting pieces. Methods HPLC meth od was performed with an Intertsil RODS column （4.6 mm x 150 mm, 5 μm） at 35℃, and the mobile phase was acetonitrile-0.1% H3PO4 （ 10：90 v/v） mixture. The flow rate was 1.0 mL. min-l. The loganin was detected at 240 nm. Results The linear range was between 0.421 2 x 4.212 μg,r= 1.000 0（n=6）.The average recovery and RSD were 98.9% ,1.6% ,respectively. Conclusion This method is simple, accurate and reliable, which can be used for the determination of loganin in Radix Dipsaci decocting pieces. |
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| Keywords: | HPLC Radix Dipsaci loganin |
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