| nm23-H1抑制原发性肝癌细胞转移的初步机理研究 |
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| 引用本文： | 张开泰,王玉芝,韩本立.nm23-H1抑制原发性肝癌细胞转移的初步机理研究[J].中国普外基础与临床杂志,1999,6(5):273-275. |
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| 作者姓名： | 张开泰 王玉芝 韩本立 |
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| 作者单位： | 1. 军事医学科学院二所四室,北京,100850
2. 第三军医大学西南医院肝胆外科 |
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| 摘 要： | 为了更深入阐明ｎｍ２３－Ｈ１抑制原发性肝癌转移的作用机理，观察ｎｍ２３－Ｈ１表达状况对肝癌细胞浸润相关因素的影响。本实验采用基因转染手段将外源ｎｍ２３－Ｈ１全长ｃＤＮＡ导入肝癌细胞并以此观察细胞体外浸润能力，细胞内游离Ｃａ＾２＋以及Ｎ－ｒａｓ基因ｍＲＮＡ表达的变化。
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| 关 键 词： | 原发性 肝癌 nm23－H1基因 肿瘤转移 病理学 |
A PRIMARY EXPERIMENTAL STUDY OF SUPPRESSIVE EFFECTS OF NM23-H1 ON METASTASIS OF PRIMARY HEPATOCELLULAR CARCINOMA CELLS |
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| Zhang Kaitai,Wang Yuzhi,Han Benli.A PRIMARY EXPERIMENTAL STUDY OF SUPPRESSIVE EFFECTS OF NM23-H1 ON METASTASIS OF PRIMARY HEPATOCELLULAR CARCINOMA CELLS[J].Chinese Journal of Bases and Clinics In General Surgery,1999,6(5):273-275. |
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| Authors: | Zhang Kaitai Wang Yuzhi Han Benli |
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| Abstract: | For an advanced elucidation of mechanisms of nm23 H1 suppressive effects on metastasis of primary hepatocellular carcinoma (HCC), it is necessary to investigate the correlation between nm23 H1 expression and relative factors involved in the HCC invasion. In present report, full length cDNA of nm23 H1 was subcloned into pBKCMV vector and transfected into HCC cell line to observe its effects on invasion, cytosolic free Ca 2 and N ras mRNA expression. The results showed that lower expression of N ras and higher cytosolic free Ca 2 in transfected cell line were detected, while the potential of invasion was depressed. It suggests that the suppressive effects on HCC metastasis might interact with intracellular signal transduction which is essential for stimulating cell invasion. |
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| Keywords: | Primary hepatocellular carcinoma Invasion nm23 H1 gene |
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