| 大鼠壁细胞的黏膜刮取式分离方法 |
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| 引用本文： | 孙凤蓬,宋于刚,张彦,高天明,张亚历,宋兰林.大鼠壁细胞的黏膜刮取式分离方法[J].解放军医学杂志,2003,28(9):812-813,I001. |
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| 作者姓名： | 孙凤蓬 宋于刚 张彦 高天明 张亚历 宋兰林 |
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| 作者单位： | 1. 510515,广州,第一军医大学南方医院
2. 第一军医大学生理学教研室 |
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| 基金项目： | 广东省自然科学基金 (编号 0 1 0 578)，广东省社会发展攻关计划项目 (编号 2 0 0 2C31 2 1 0 )，广州市科技攻关计划项目 (编号 2 0 0 2Z3 E0 1 31 )资助课题 |
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| 摘 要： | 采用黏膜刮取式方法，分离大鼠胃黏膜细胞。用相差显微镜、HE染色、电镜、荧光显微镜、免疫细胞化学染色等方法，对壁细胞进行观察和鉴定，并测定了壁细胞直径。结果成功地分离了大鼠胃黏膜细胞，同时证实，壁细胞在所分离的细胞中直径最大。
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| 关 键 词： | 大鼠 SD 胃黏膜 壁细胞 胃 细胞分离 |
SCRAPING METHOD FOR ISOLATION OF RAT PARIETAL CELLS |
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| Sun Fengpeng,Song Yugang,Zhang Yan et al.SCRAPING METHOD FOR ISOLATION OF RAT PARIETAL CELLS[J].Medical Journal of Chinese People's Liberation Army,2003,28(9):812-813,I001. |
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| Authors: | Sun Fengpeng Song Yugang Zhang Yan |
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| Institution: | Sun Fengpeng,Song Yugang,Zhang Yan et al. Nanfang Hospital,First Military Medical University,Guangzhou 510515,China |
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| Abstract: | Isolation of rat gastric mucosal cells was performed with scraping method followed by protease and collagenase digestion. Parietal cells were observed and identified with phase-contrast microscopy, light microscopy with hematoxylin and eosin staining, electron microscopy, fluorescence microscopy, and also immunocytochemistry technique. The diameters of parietal cells were measured. The results showed that the mucosal cells were successfully isolated, and it was confirmed that the parietal cells were the biggest cells among all the mucosal cells. |
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| Keywords: | rats gastric mucosa parietal cells gastric cell separation |
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