吉非罗齐对游离脂肪酸和脂肪型脂肪酸结合蛋白在血脂异常兔中影响

目的观察吉非罗齐短期干预对高胆固醇血症兔血清游离脂肪酸（FFA）及脂肪组织分泌脂肪细胞型脂肪酸结合蛋白（AFABP）的影响,并阐明其可能机制。方法 15只新西兰大白兔随机分为3组,每组5只。①对照组：给予普通饮食12周。②高胆固醇组：给予高胆固醇饲料12周。③吉非罗齐组：在饲以高胆固醇饲料8周的基础上给予吉非罗齐200 mg·kg^-1·d^-1 4周。酶联免疫吸附测定（ELISA）法检测血清FFA、AFABP水平,应用反转录聚合酶链反应（RT-PCR）法测定脂肪组织AFABPmRNA的表达。结果高胆固醇组和吉非罗齐组兔饲养第8周及第12周血清总胆固醇、低密度脂蛋白胆固醇、FFA水平均明显高于对照组（P〈0.01）;第12周时高胆固醇组和吉非罗齐组兔饲养血清总胆固醇、低密度脂蛋白胆固醇、FFA水平均明显高于对照组（P〈0.01）;吉非罗齐组兔第12周末体质量较第8周明显下降（P〈0.05）,同时血清和脂肪组织培养的上清液中AFABP水平明显低于高胆固醇组（P〈0.05）;高胆固醇组和吉非罗齐组脂肪组织AFABPmRNA的表达明显高于对照组（P〈0.05）,但吉非罗齐组兔脂肪组织AFABPmRNA的表达明显低于高胆固醇组（P〈0.05）。结论吉非罗齐能降低高胆固醇饮食饲养兔体质量,并降低FFA、血清及脂肪组织分泌AFABP,这一作用可能有利于肥胖及动脉粥样硬化的防治。

Influence of gemfibrozil on levels of adipocyte fatty acid-binding protein in serum of dyslipidemia rabbits

Objective To observe the effects of gemfibrozil on serum free fatty acid（FFA） concentration and adipocyte fatty acid-binding protein（AFABP） secretion by adipose tissue from hypercholesterolemia rabbits.Methods Fifteen male New Zealand white rabbits were randomly divided into three groups.①Control group was fed with normal diet for 12 weeks;②high cholesterol group was fed with high cholesterol group diet for 12 weeks;③gemfibrozil group was fed with same cholesterol diet for eight weeks supplemented with gemfibrozil 200 mg·kg^-1·d^-1 for 4 weeks.Each group had five rabbits.FFA and AFABP concentrations in serum and adipose tissue culture supernatant were measured by enzyme-linked immunosorbent assay（ELISA）.Adipose tissue was harvested for reverse transcription-polymerase chain reaction（RT-PCR） studies.Results After 8 and 12 weeks,rabbits from high cholesterol group and gemfibrozil group showed higher serum levels of total cholesterol,low density lipoprotein cholesterol and FFA than those of control group（P〈0.01）.Gemfibrozil treatment significantly decreased body mass of rabbits in the twelfth week than in the eighth week（P〈0.05）.AFABP concentrations in serum and in culture supernatants of adipose tissue were decreased in gemfibrozil group compared with high-cholesterol group at the end of the twelfth week（P〈0.05）.The mRNA levels of AFABP in adipose tissue were increased in high cholesterol group and gemfibrozil group compared with control group（P〈0.05）;but the mRNA levels of AFABP in adipose tissue was decreased in gemfibrozil group compared with high-cholesterol group（P〈0.05）.Conclusion The study indicated that gemfibrozil reduced body mass and reduced FFA and AFABP serum concentration and adipose tissue secretion of hypercholesterolemia rabbits.These effects might be of significance for the treatment of atherosclerosis and of obesity prevention.