NLRP3在继发性甲状旁腺功能亢进症中的表达及临床意义

目的:探讨NOD样受体家族蛋白3(NLRP3)炎性小体在慢性肾脏病继发性甲状旁腺功能亢进症(SHPT)中的表达及临床意义.方法:应用HE染色观察SHPT病人异常增生的甲状旁腺镜下形态;采用ELISA法检测正常人和SHPT病人血清中NLRP3水平;免疫组织化学SP法检测NLRP3在正常和异常增生的甲状旁腺组织中的表达,并...

Expression of NLRP3 in secondary hyperparathyroidism and its clinical significance

ObjectiveTo investigate the expression and clinical significance of NOD-like receptor family pyrin domain containing 3(NLRP3) in secondary hyperparathyroidism(SHPT).MethodsHE staining was used to observe the difference of parathyroid gland in normal people and SHPT patients.ELISA was used to detect the serum level of NLRP3 in normal people and SHPT patients.Immunohistochemistry of SP method was used to detect the expression of NLRP3 in normal and hyperplastic parathyroid tissue, and the relationship between NLRP3 expression and SHPT clinical-pathological characteristics was analyzed.ResultsHyperplastic parathyroid glands in SHPT patients manifested as diffuse and adenoma-like hyperplasia, which was obviously different from that of normal, and the serum level of NLRP3 in SHPT patients was significantly higher than that of normal people(P < 0.01).The positive rate of NLRP3 in abnormal hyperplastic parathyroid tissue in SHPT patients was significantly higher than that in normal parathyroid tissue(P < 0.01).The expression of NLRP3 in SHPT patients had no correlation with gender, age, dialysis method or dialysis age(P>0.05).ConclusionsNLRP3 may be involved in the occurrence and development of SHPT, and its high expression is expected to become an important indicator for the early diagnosis and prognostic evaluation of SHPT.