NFATc1、MAPK及CTGF在心房颤动病人外周血中的表达及临床意义

目的探讨活化T细胞核因子c1(NFATc1)、丝裂原激活蛋白激酶(MAPK)及结缔组织生长因子(CTGF)在心房颤动病人外周血中的表达及其临床意义。 方法选取心房颤动病人139例作为心房颤动组(房颤组),选取同期窦性心律的健康体检者105名作为对照组。采用酶联免疫吸附试验检测2组血清中NFATc1、MAPK水平,Western blotting检测外周血单个核细胞中CTGF的表达。比较2组受试者血生化指标及心脏彩超检查指标。分析NFATc1、MAPK与左心室射血分数(LVEF)、左心房内径(LAD)的相关性,探讨心房颤动发病的危险因素。 结果房颤组丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、NFATc1、MAPK、CTGF水平及LAD均高于对照组(P < 0.05~P < 0.01),LVEF明显低于对照组(P < 0.01)。Pearson相关分析显示,血清NFATc1、MAPK水平与LVEF均呈明显负相关关系(r=-0.195、-0.189,P < 0.01),与LAD呈明显正相关关系(r=0.288、0.244,P < 0.01)。二元logistics回归分析结果显示,NFATc1、MAPK水平升高及LAD增大均为心房颤动发病的危险因素(P < 0.01)。 结论心房颤动病人血清中NFATc1、MAPK、CTGF水平的升高可能与心肌纤维化有关。

Expression and clinical significance of NFATc1 ,MAPK and CTGF in peripheral blood of patients with atrial fibrillation

ObjectiveTo investigate the expression and clinical significance of nuclear factor of activated T cells c1(NFATc1), mitogen-activated protein kinase(MAPK), and connective tissue growth factor(CTGF) in peripheral blood of patients with atrial fibrillation. MethodsA total of 139 patients with atrial fibrillation were selected as the atrial fibrillation group, and 105 healthy examinees with sinus rhythm were selected as the control group.The levels of NFATc1 and MAPK in serum were detected by enzyme-linked immunosorbent assay, and the expression of CTGF in peripheral blood mononuclear cells was determined by Western blotting.The blood biochemical indexes and cardiac color Doppler ultrasound indexes in the two groups were compared.The correlation between NFATc1, MAPK and left ventricular ejection fraction(LVEF), left atrial diameter(LAD) was analyzed, and the risk factors of atrial fibrillation were explored. ResultsThe levels of alanine aminotransferase, aspartate aminotransferase, NFATc1, MAPK, CTGF and LAD in the atrial fibrillation group were higher than those in the control group(P < 0.05 to P < 0.01), and the level of LVEF was significantly lower than that in the control group(P < 0.01).Pearson correlation analysis showed that the levels of serum NFATc1 and MAPK were significantly negatively correlated with LVEF(r=-0.195, -0.189, P < 0.01), and positively correlated with LAD(r=0.288, 0.244, P < 0.01).The binary logistic regression analysis showed that the increased levels of NFATc1, MAPK and LAD were the risk factors of atrial fibrillation(P < 0.01). ConclusionsThe increase of serum NFATc1, MAPK and CTGF levels in patients with atrial fibrillation may be related to myocardial fibrosis.