| MiR-514与胃癌细胞凋亡的关系及机制 |
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| 引用本文： | 檀碧波,李勇,赵群,范立侨,狄岩,温转. MiR-514与胃癌细胞凋亡的关系及机制[J]. 转化医学杂志, 2019, 8(2): 80-84 |
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| 作者姓名： | 檀碧波 李勇 赵群 范立侨 狄岩 温转 |
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| 作者单位： | 河北医科大学第四医院外三科,河北医科大学第四医院外三科,河北医科大学第四医院外三科,河北医科大学第四医院外三科,河北省医学情报研究所,河北医科大学第四医院质量控制办公室 |
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| 基金项目： | 河北省自然科学基金面上项目(H2016206173)；河北省科技支撑项目(14277779D)；河北省卫生和计划生育委员会重点科技研究计划(20160176) |
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| 摘 要： | 目的 探讨微小RNA-514（microRNA-514,miR-514）与胃癌细胞凋亡的关系及机制。方法 实时定量PCR检测80例胃癌组织及癌旁正常胃黏膜组织中miR-514的表达情况，分析miR-514与患者各病理参数之间的关系。应用miR-514模拟物转染人胃癌细胞株MGC803，检测转染前后细胞凋亡率及凋亡相关基因的情况。结果 胃癌组织中miR-514水平（0.3619±0.2103）明显低于癌旁正常胃黏膜（0.6943±0.3032）（t=-8.0573；P<0.001）；miR-514表达与肿瘤的淋巴结转移有关（t=-2.5620,P=0.012 3）；miR-514模拟物转染人胃癌细胞株MGC803后细胞的活性明显降低而凋亡率明显增高（P<0.05）；转染后凋亡相关基因Bcl-2、xIAP的mRNA和蛋白表达明显降低，而Bax的mRNA和蛋白表达明显增高（P<0.05）。结论 胃癌组织中miR-514的表达减弱，miR-514可能通过调节一些凋亡基因表达而参与肿瘤的凋亡过程。
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| 关 键 词： | 胃癌；微小RNA 514；凋亡；病理参数 |
Relationship between miR-514 and apoptosis of gastric cancer and related mechanism |
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| TAN Bibo,LI Yong,ZHAO Qun,FAN Liqiao,DI Yan and WEN Zhuan. Relationship between miR-514 and apoptosis of gastric cancer and related mechanism[J]. Translational Medicine Journal, 2019, 8(2): 80-84 |
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| Authors: | TAN Bibo LI Yong ZHAO Qun FAN Liqiao DI Yan WEN Zhuan |
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| Affiliation: | The Third Department of Surgery, the Fourth Hospital of Hebei Medical University, Shijiazhuang Hebei 050011, China,The Third Department of Surgery, the Fourth Hospital of Hebei Medical University, Shijiazhuang Hebei 050011, China,The Third Department of Surgery, the Fourth Hospital of Hebei Medical University, Shijiazhuang Hebei 050011, China,The Third Department of Surgery, the Fourth Hospital of Hebei Medical University, Shijiazhuang Hebei 050011, China,Hebei Provincial Institute of Medical Science Information, Shijiazhuang Hebei 050071, China; and Quality Control Office, the Fourth Hospital of Hebei Medical University, Shijiazhuang Hebei 050011, China |
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| Abstract: | Objective To investigate the relationship between microRNA-514 (miR-514) and apoptosis of gastric cancer and related mechanism. Methods Expression of miR-514 in gastric cancer tissues and cancer-adjacent normal tissues from 80 patients were detected with real-time PCR, and relationship between miR-514 and clinicopathological parameters was investigated. miR-514 mimics was utilized to transfected into gastric cancer cell line MGC803, then variation of apoptosis rate and related genes was detected. Results Level of miR-514 was lower in gastric cancer tissues (0.3619±0.2103) than that in cancer-adjacent normal tissues (0.6943±0.3032) (t=-8.0573, P<0.001). Expression of miR-514 was related with lymph node metastasis (t=-2.5620, P=0.0123). After transfection of miR-514 mimics, cell activity was decreased, while apoptotic rate of MGC803 increased significantly(P<0.05); mRNA and protein of Bcl-2, xIAP were decreased significantly, while Bax increased significantly (P<0.05). Conclusion Reduction of miR-514 existed in gastric cancer tissues, which may be invovled in apoptosis of gastric cancer cells by regulating apoptosis related genes. |
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| Keywords: | Gastric cancer MicroRNA-514 (miR-514) Apoptosis Clinicopathological parameters |
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