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| 肺癌组织、支气管肺泡灌洗液及痰标本中p16基因甲基化特异性PCR检测 | |
| 引用本文： | 杨丽萍,张舒林,刘旺根,何苡. 肺癌组织、支气管肺泡灌洗液及痰标本中p16基因甲基化特异性PCR检测[J]. 郑州大学学报(医学版), 2003, 38(5): 734-736 |
| 作者姓名： | 杨丽萍 张舒林 刘旺根 何苡 |
| 作者单位： | 1. 河南中医学院生物化学与分子生物学学科,郑州,450003 2. 河南省胸科医院分子生物学研究室,郑州,450003 3. 河南中医学院组织学与胚胎学学科,郑州,450003 4. 河南省人民医院胸外科,郑州,450003 |
| 基金项目： | 河南省科技攻关资助项目 004022000 |
| 摘 要： | 目的：探讨肺癌组织、支气管灌洗液及痰标本中p16基因甲基化特异性PCR检测的临床应用价值。方法：选取56例肺部疾病住院患者手术切除的病变肺组织和相应的支气管肺泡灌洗液(BALF)及痰标本，其中32例为肺癌，24例为良性肺部疾病。标本经一般处理，PCR扩增后，产物经电泳EB染色，紫外灯下观察。结果：32例肺癌组织标本中，14例(43．8％)在p16基因启动子区域呈现异常甲基化，其中9例(64．3％)在相应的BALF中检出甲基化存在，5例(35．8％)在相应的痰标本中也检出甲基化存在。24例良性肺部疾病，其中肺囊肿10例，肺结核14例，无论在手术切除标本还是BALF和痰标本中均未检出p16基因甲基化存在。结论：MSP技术对肺癌患者BALF及痰标本中p16基因的异常甲基化检测具有高度特异性，是一项很有潜力的肺癌早期诊断新技术。 |
| 关 键 词： | 甲基化特异性PCR 肺肿瘤 诊断 组织 支气管灌洗液 痰标本 |
| 修稿时间： | 2003-01-16 |
Detection of aberrant methylation of p16 gene in lung tumor tissues,corresponding bronchoalveolar lavage fluid, and sputum by methylation-specific PCR |
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| YANG Liping ),ZHANG Shulin ),LIU Wanggen ),HE Yi ) )Subject of Biochemistry and Molecular Biology,Henan Chinese Traditional Medical College,Zhengzhou ). Detection of aberrant methylation of p16 gene in lung tumor tissues,corresponding bronchoalveolar lavage fluid, and sputum by methylation-specific PCR[J]. Journal of Zhengzhou University: Med Sci, 2003, 38(5): 734-736 | |
| Authors: | YANG Liping ) ZHANG Shulin ) LIU Wanggen ) HE Yi ) )Subject of Biochemistry Molecular Biology Henan Chinese Traditional Medical College Zhengzhou ) |
| Affiliation: | YANG Liping 1),ZHANG Shulin 2),LIU Wanggen 3),HE Yi 4) 1)Subject of Biochemistry and Molecular Biology,Henan Chinese Traditional Medical College,Zhengzhou 450003 2)Department of Molecular Biology,Chest Hospital of Henan Province,Zhengzhou 450003 3)Subject of Histology and Embryology,Henan Chinese Traditional Medical College,Zhengzhou 450003 4)Department of Thoracic Surgery,Henan Provincial People's Hospital,Zhengzhou 450003 |
| Abstract: | Aim:To explore the clinical value of methylation specific PCR (MSP) on the diagnosis of Lung cancer. Methods:Pathological tissues taken in operation, corresponding bronchoalveolar lavage fluid (BALF), and sputum were obtained from 56 hospitalized patients, among whom 32 were lung cancer, and 24 were benign lung diseases.After DNA extracting and PCR amplification, the PCR products were stained with EB and observed under ultraviolet lamp.Results:Among 32 lung cancer tissues,14(43.8%) were found abnormal methylation in promoter area of p16 gene,9(64.3%), in corresponding BALF, and 5(35.8%), in corresponding sputum. While among 24 cases of benign lung diseases no p16 gene methylation was found in pathological tissues taken in operation, corresponding BALF or sputum. Conclusion:MSP has specificity on the detection of abnormal methylation of P16 gene in BALF and sputum of patients with lung cancer. This suggests that MSP might be a promising technique for lung cancer early diagnosis |
| Keywords: | methylation specific PCR lung neoplasm diagnosis |
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