脑缺血大鼠脑脊液对神经干细胞存活及分化的影响

目的：探讨脑缺血大鼠脑脊液对新生大鼠海马神经干细胞生存及分化的影响。方法：将脑缺血大鼠脑脊液加入神经干细胞的培养液中，观察神经干细胞存活及贴壁细胞团的分化．并应用免疫细胞化学技术对神经干细胞分化情况进行鉴定。结果：悬浮培养细胞实验组较对照组有明显差异；贴壁培养细胞分化的细胞数量较对照组明显增多。结论：脑缺血大鼠脑脊液可促进体外培养神经干细胞存活，并且促进神经干细胞向神经元和星形胶质细胞分化。

Effects of the ischemic cerebrospinal fluid on survival and differentiation of hippocampal neural stem cells in rats

Objective: To investigate the effect of the rat cerebrospinal fluid on the survival and differentiation of the neural stem cells isolated from the hippocampus of neonatal rats .Methods:The neural stem cells were cultured in the media with the cerebrospinal fluid of rat brain ischemia .The survival of the neural stem cells was observed with phaze microscope and the differentiation of neural stem cells was identified by the immunocytochemical technique.Results:The number of survival neural stem cells in experimental group was significantly higher than that of the control.In the meantime,the number of the cells migrating from the neurospheres after the attachment was significantly increased compared with the control.Conclusion:The cerebrospinal fluid in rat brain ischemia not only can promote the survival and growth of neural stem cells,but also promote the differentiation of the stem cells into neurons and astrocytes .