玉枢含片对急性咽炎大鼠血清白介素-6及肿瘤坏死因子等指标的影响

观察玉枢含片治疗急性咽炎的大鼠血清白介素-6及肿瘤坏死因子等指标变化,以进一步探讨其治疗机理.将50只Wistar大鼠随机分为正常对照组、玉枢含片高剂量组、玉枢含片低剂量组、模型对照组及草珊瑚组,每组10只,采用浓氨水喉头喷雾诱发的大鼠急性咽炎模型,于第4～7天分别用喉头喷雾器给大鼠咽部喷蒸馏水、高剂量玉枢含片液(1.6 g/kg)、低剂量玉枢含片液(0.4 g/kg)、草珊瑚含片液(4.8 g/kg).实验第7日取5组动物的股静脉血检测血清白细胞介素-6(IL-6)及肿瘤坏死因子(TNF)含量.同时,取咽部粘膜及粘膜下组织分成两份分别进行病理形态学及超微结构观察.结果示高、低剂量玉枢含片均能有效降低模型大鼠血液淋巴细胞百分比,降低模型大鼠血清中白细胞介素-6(IL-6)、肿瘤坏死因子(TNF)含量,改善模型大鼠咽部组织病理形态及超微结构.说明玉枢含片在缓解咽部症状、改善咽部病理组织形态及超微结构方面,具有独特优势.

Effects of Yushu Buccal Tablets on Serum Interleukin-6 and Tumor Necrosis Factor in the Rats with Acute Pharyngitis

In order to investigate the mechanism of Yushu Buccal Tablets (YBT) in treating acute pharyngitis, effects of YBT on the serum interleukin-6 (IL-6) and tumor necrosis factor (TNF) were observed in the rats with acute pharyngitis. 50 Wistar rats were divided into 5 groups, namely the normal control group, large dose YBT group, small dose YBT group, model control group, Caoshanhu group, and each group consisted of 10 animals. The rat model of acute pharyngitis was induced by spraying the larynx with strong ammonia water. Distilled water, large dose of YBT solution (1.6 g/kg), small dose of YBT solution (0.4 g/kg) and Caoshanhu Buccal Tablets solution (4.8 g/kg) were respectively given to the animals in each corresponding group. On the 7th day of the experiment, the levels of serum IL-6 and TNF were detected in the blood of the femoral vein of the animals in the 5 groups respectively, and at the same time, the pharyngeal mucosal and submucosal tissues of the animals were also examined pathomorphologically and ultrastructurally. The results showed that both large dose and small dose of YBT could effectively decrease the percentage of blood lymph cells and the levels of serum IL-6 and TNF, and improve the pathologic picture and ultrastructure of the pharyngeal tissues in the model animals.