| 鱼藤素作用K562细胞Topo Ⅰ表达的探讨 |
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| 引用本文： | 陈卫华,陈燕,崔国惠. 鱼藤素作用K562细胞Topo Ⅰ表达的探讨[J]. 中国肿瘤临床, 2007, 34(3): 121-124 |
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| 作者姓名： | 陈卫华 陈燕 崔国惠 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院血液病研究所,武汉市,430022 |
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| 摘 要： | 目的：探讨鱼藤素对DNA拓扑异构酶TopoⅠ表达的影响，进一步明确鱼藤素抑制肿瘤细胞增殖的作用机制。方法：流式细胞术及RT-PCR法分析鱼藤素作用K562细胞后TopoⅠ蛋白及mRNA含量的变化。结果：流式细胞术分析结果发现TopoⅠ蛋白的平均荧光强度随着鱼藤素作用浓度的增高而逐渐减少，与空白对照组比较有显著差异（P〈0．05）；RT-PCR半定量检测结果显示，鱼藤素作用K562细胞引起TopoⅠmRNA含量减少，并与其浓度正相关。结论：鱼藤素可通过抑制肿瘤细胞内TopoⅠ而发挥作用。
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| 关 键 词： | 鱼藤素 Topo Ⅰ K562细胞株 |
| 文章编号： | 1000-8179（2007）03-0121-04 |
| 收稿时间： | 2006-08-14 |
| 修稿时间： | 2006-08-14 |
The Effect of Deguelin on Expression of TopoⅠ in K562 Cells |
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| Chen Weihua,Chen Yan,Cui Guohui. The Effect of Deguelin on Expression of TopoⅠ in K562 Cells[J]. Chinese Journal of Clinical Oncology, 2007, 34(3): 121-124 |
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| Authors: | Chen Weihua Chen Yan Cui Guohui |
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| Affiliation: | Institute of Hematology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan |
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| Abstract: | Objective: To investigate the effect of deguelin on the expression of TopoI in K562 cells. Methods: Changes in TopoI mRNA and protein levels were observed by RT-PCR and flow cytometry. Results: Flow cytometry showed that gradually decreasing expression of the TopoI protein was induced by an increase of deguelin protein. RT-PCR analysis also showed that deguelin reduced TopoI mRNA in a dose-dependent manner. Conclusion: Deguelin can inhibit the expression of TopoI protein. The anti-proliferative activity of deguelin is related to TopoI protein expression in K562 cells. |
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| Keywords: | Deguelin Topoi Cell line K562 |
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