Ultrastructural evidence for an innervation of epithelial enterochromaffine cells in the guinea pig duodenum |
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Authors: | Jan M. Lundberg,Annica Dahlstr m,Anders Bylock,H kan Ahlman,G sta Pettersson,Inger Larsson,Hans-Arne Hansson,Jan Kewenter |
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Affiliation: | Jan M. Lundberg,Annica Dahlström,Anders Bylock,Håkan Ahlman,Gösta Pettersson,Inger Larsson,Hans-Arne Hansson,Jan Kewenter |
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Abstract: | The innervation of the duodenal enterochromaffine cells (E.C.) of the guinea pig was studied at the electronmicroscopic level. Pretreatment with 5-OH-dopamine was performed to visualize catecholaminergic (CA) nervous elements. Near the basement membrane of all examined E.C. in the crypts, bundles of unmyelinated nerve processes were observed, only partly ensheathed in a Schwann cell cover. At least 4 types of processes could be observed. 1) Boutons containing only small clear vesicles, probably cholinergic fibres; 2) boutons with small clear vesicles, and in addition large (200 nm) granules with a dense matrix (P-type-fibres); 3) boutons with small electron-dense vesicles, probably CA-fibres; and 4) processes with few vesicles but having the appearance of dendrites. No typical synaptic arrangements were observed, but the minimal distance between the E.C. and the nerve bundles was 150 to 250 nm, thus well within the functional limits of the “autonomic gap”. Thus, epithelial E.C. may be influenced by several types of nervous elements, including CA-fibres. |
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Keywords: | enterochromaffine cells guinea pig duodenum electron microscopy adrenergic nerve terminals bundles of nerve processes |
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