人外周血分离树突状细胞前体分离培养及其分化成熟过程中生物特征的变化

用从人外周血分离、纯化、扩增树突状细胞的方法,对树突状细胞成熟过程中的功能分化作初步探讨.以酵母菌、HRP吞噬实验测试树突状细胞的内吞能力;以同种混合淋巴细胞反应检测细胞的免疫刺激能力.结果显示,在GM-CSF或GM-CSF与IL-4联合培养2～3天后,树突状细胞无或仅有极弱的吞噬酵母菌能力,但能活跃内吞HRP.培养5天的树突状细胞内吞HRP能力减弱.但能有效的刺激T细胞增殖,GM-CSF与IL-4联合作用后的树突状细胞,其内吞HRP能力及刺激T细胞能力均强于单用GM-CSF培养的树突状细胞.由此可见,树突状细胞在分化成熟的不同阶段表现出不同的功能,GM-CSF与IL-4联合培养能明显增强树突状细胞功能.

BIOLOGICAL CHARACTERISTIC CHANGES OF DENDRITIC CELL FROM HUMAN PERIPHERAL BLOOD DURING ITS CULTURE DIFFERENTIATION AND MATURATION

The differentiation of dendritic cells (DC) function during its maturation was studied by using the method of isolation, purification and propagation of DC precursors from human periph-eral blood. The cultured DCs were incubated with yeasts and HRP respectively, and the endocy-tosis capacities of DCs were measured. Allogenic mixed lymphocyte reactions (MLR) were per-formed to evaluate the allo-stimulating activity of DCs. After 2 or 3 days' culture with GM-CSF or GM-CSF plus IL-4, DCs showed very weak or no endocytosis for yeast but uptook HRP ac-tively. On the 5th day, the endocytosis capacity for HRP of DCs became impotent, but allogenic MLR stimulatory activity increased markedly. The endoeytosis ability and stimulatory activity of DCs cultured with GM-CSF plus IL-4 were more active than those with GM-CSF. The results re-vealed that DCs on different phases of differentiation exhibited different functions, and that a combination of GM-CSF plus IL-4 could greatly enhance the functional capacity of DCs.