| 冬凌草甲素诱导HL-60细胞凋亡 |
| |
| 引用本文： | 王筠,刘清芸,华海婴,叶启霞,张予,张覃沐. 冬凌草甲素诱导HL-60细胞凋亡[J]. 中国药理学通报, 2001, 17(4): 402-404 |
| |
| 作者姓名： | 王筠 刘清芸 华海婴 叶启霞 张予 张覃沐 |
| |
| 作者单位： | 1. 河南省医学科学研究所药理室,
2. 河南医科大学生物遗传教研室, |
| |
| 基金项目： | 国家自然科学基金资助项目,No 39700178 |
| |
| 摘 要： | 目的 研究冬凌草甲素诱导人白血病HL 6 0细胞凋亡的作用。方法 形态学观察 ,DNA凝胶电泳及流式细胞术。结果 冬凌草甲素能显著地诱导HL 6 0细胞发生凋亡 ,其作用呈明显的浓度效应关系和时间依赖性。形态学观察可见凋亡小体的形成 ,琼脂糖凝胶电泳可见明显的DNA梯带 ;流式细胞仪检测到G1亚峰。结论 冬凌草甲素能诱导HL 6 0细胞凋亡 ,并与其细胞杀伤活性相互平行 ,提示冬凌草甲素的抗癌活性与诱导肿瘤细胞凋亡相关
|
| 关 键 词： | 冬凌草甲素 细胞凋亡 HL-60细胞 |
| 文章编号： | 1001-1978(2001)04-0402-03 |
Oridonin induces apoptosis in human leukemia HL-60 cells |
| |
| WANG Jun,LIU Qing Yun,HU Hai Ying,YE Qi Xia,ZHANG Yu,ZHANG Tan Mu. Oridonin induces apoptosis in human leukemia HL-60 cells[J]. Chinese Pharmacological Bulletin, 2001, 17(4): 402-404 |
| |
| Authors: | WANG Jun LIU Qing Yun HU Hai Ying YE Qi Xia ZHANG Yu ZHANG Tan Mu |
| |
| Abstract: | AIM To study oridonin induced apoptosis in human leukemia HL 60 cells. METHODS Cell morphology, agarose gel electrophoresis and flow cytometry were used. RESULTS Oridonin had significant apoptotic inductive effect on HL 60 cells in both concentration and time dependent fashion. The marked morphological changes including condensed chromatin, nuclear fragmentation and apoptotic body were observed. Typical DNA ladder in agarose gel electrophoresis and pre G 1 peak in flow clytometric analysis were also observed in the cells exposed to Ori. CONCLUSION Oridonin can induce apoptosis in human leukemia HL 60 cells. |
| |
| Keywords: | Oridonin HL 60 cells apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
