γ-氨基丁酸对胰腺癌SW1990细胞生长及VEGF表达的影响

目的观察γ-氨基丁酸（GABA）对胰腺癌SW1990细胞生长及血管内皮生长因子（VEGF）表达的影响。方法采用MTT法和流式细胞术（FCM）检测GABA对胰腺癌SW1990细胞增殖、细胞凋亡和细胞周期的影响，放射免疫分析法检测cAMP含量，RT—PCR检测VEGF mRNA表达，Western blot检测VEGF蛋白含量。结果GABA促进胰腺癌SW1990细胞生长，抑制细胞凋亡，同时促进细胞内cAMP增加。随着GABA浓度增加（80～320μmol／L），VEGF mRNA及蛋白表达明显增加。结论GABA影响胰腺癌细胞增殖、凋亡和血管形成，可能参与调节肿瘤的生长与转移。

Effect of GABA on cell growth and VEGF expression of pancreatic cancer cell line SW1990

[Objective] To observe the effect of GABA on proliferation, apoptosis and VEGF expression of pancreatic cancer cell line SW-1990. [Methods] The effect of GABA on proliferation and apoptosis, cell cycle of pancreatic cancer cell line SW-1990 was mearsured by MTT assay and flow cytometry analysis, respectively. Radioimmunoassay was used to measure the intracellular cAMP content. Expressions of VEGF mRNA and protein were evaluated by semi-quantity RT-PCR and Western-blot analysis. [Results] GABA promoted the proliferation of SW-1990 cells and influenced the distribution of cell cycle. The apoptosis rate of SW-1990 cells significantly decreased from 27.5% to 5. 4% and the intracellular cAMP content increased with the increase of GABA concentration in a dose-dependent manner. After treatment with GABA, expressions of VEGF mRNA and protein were increased in pancreatic cancer cells. [Conclusions] GABA can influence the proliferation, apoptosis and angiogenesis of SW-1990 cells. GABA may be have a high correlation with the regulation of growth and metastasis of turnout.