The Effect of the Addition of Adenine and Nucleosides at the Beginning of Storage on the Concentrations of Phosphates of Human Erythrocytes during Storage in Acid-Citrate-Dextrose and Citrate-Phosphate-Dextrose

Bloods collected in ACD and CPD preservatives, supplemented with varying amounts of adenine, inosine, adenosine, and guanosine, were stored anaerobically and aerobically at 4 C for six weeks. At two-week intervals, phosphorylated carbohydrate intermediates of washed red cells were assayed by ion-exchange chromatography. The 2,3-diphospho-glycerate values were higher in the CPD or CPD-supplemented than in the ACD bloods which were at lower pH levels. Generally, the ATP and the sum of adenylate (ΣAd) concentrations were maintained at higher levels for longer periods when bloods were collected in more acidic solutions. The highest ATP and ΣAd levels were observed in ACD-adenine (.075, 0.10 and 0.25 mmole/100 ml blood) and in CPD-adenine (0.5 mmole) anaerobically-stored bloods. Relatively high ATP and ΣAd levels were maintained in blood collected in ACD-inosine -+ adenine + guanosine and in inosine-adenine preservatives. Lower values were obtained with ACD-inosine, ACD-adenosine and ACD-adeno-sine + adenine, in the order listed. The ADP values remained relatively constant during storage. An appreciable increase in AMP concentration was observed only in the adenine-supplemented bloods. The IMP concentration increased in all stored bloods and was particularly marked in the presence of adenosine and least in the adenine-supplemented bloods.