| 全反式维甲酸可增强大鼠系膜细胞uPA和MMP—2表达 |
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| 引用本文： | 张农,刘琛,林文生,郭慕依. 全反式维甲酸可增强大鼠系膜细胞uPA和MMP—2表达[J]. 复旦学报(医学版), 2001, 28(4): 281-283 |
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| 作者姓名： | 张农 刘琛 林文生 郭慕依 |
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| 作者单位： | 复旦大学基础医学院病理学教研室 |
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| 基金项目： | 上海市科技发展基金(98QB14007)和卫生部优秀青年科技人才专项基金项目及CMB基金资助 |
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| 摘 要： | 目的 研究全反式维甲酸对体外培养大鼠系膜细胞uPA和MMP－2表达的影响，探讨全反式维甲酸抗肾小球硬化的机制。方法 分别应用Northern blot、Western blot和酶谱法检测全反式维甲酸对培养大鼠系膜细胞uPA和MMP－2表达的影响。结果 ATRA可促进系膜细胞uPA和MMP-2蛋白表达，提高其酶活性，并增强MMP－2mRNA表达。结论 ATRA可增强大鼠系膜细胞的uPA和MMP－2表达，为进一步阐明ATRA拮抗肾小球硬化机制提供了直接的实验依据。
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| 关 键 词： | 全反式维甲酸 系膜细胞 尿激酶型纤溶酶原激活物 基质金属蛋白酶－2 ATRA MsC |
| 修稿时间： | 2000-12-27 |
All-trans Retinoic Acid Upregulates the Expression of uPA/MMP-2 in Cultured Rat Mesangial Cells |
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| ZHANG Nong,LIU Chen,LIN Wen sheng,GUO Mu yi. All-trans Retinoic Acid Upregulates the Expression of uPA/MMP-2 in Cultured Rat Mesangial Cells[J]. Fudan University Journal of Medical Sciences, 2001, 28(4): 281-283 |
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| Authors: | ZHANG Nong LIU Chen LIN Wen sheng GUO Mu yi |
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| Abstract: | Purpose To study the effects of all trans retinoic acid on the expression of uPA/MMP?2 in cultured rat mesangial cells, and to elucidate the mechanism by which all trans retinoic acid prevents age related progressive glomemular sclerosis. Methods The mRNA, protein and enzymatic activity of uPA/MMP?2 were detected by Northern blot analysis, Western blot analysis and Zymography assay, respectively. Results All trans retinoic acid upregulated the expression of uPA/MMP?2 protein, enhanced the enzymatic activity of uPA/MMP?2. Thus all trans retinoic acid elevated the level of MMP?2 mRNA in cultured mesangial cells. Conclusions All trans retinoic acid upregulated the expression of uPA/MMP?2 in cultured rat mesangial cells. It is valuable for explaining the role of all trans retinoic acid in preventing age related glomerulsclerosis. |
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| Keywords: | all trans retinoic acid rat mesangial cell uPA matrix metalloproteinase 2 |
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