Determination of urea in milk based on N-bromosuccinimide–dichlorofluorescein postchemiluminescence method |
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Institution: | Institute of Analytical Science, Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi''an, Shaanxi 710069, China |
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Abstract: | A new postchemiluminescence (PCL) reaction was observed when urea was injected into the reaction mixture after the CL reaction of N-bromosuccinimide and dichlorofluorescein. A possible reaction mechanism was proposed based on the studies of the CL kinetic characteristics, CL spectra, fluorescence spectra, and other experiments. A new flow injection CL method for the determination of urea was established based on the PCL reaction. The relative standard deviation for the determination of urea was 1.3% (n = 11, c = 5.0 × 10?8 g/mL). The CL intensity responded linearly to the concentration of urea in the range 2.0 × 10?9–1.0 × 10?6 g/mL (r = 0.9992). The detection limit was 7 × 10?10 g/mL. The method had been applied to the determination of urea in milk with satisfactory results. |
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Keywords: | dichlorofluorescein milk postchemiluminescence urea |
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