广西地区人群AGT基因多态性与2型糖尿病肾病关系的研究

目的]探讨血管紧张素原(AGT)基因M235T及T174M多态性是否与广西地区人群2型糖尿病肾病(DN)的发病有关。方法]243例研究对象分为2型糖尿病病例组(DM组,n=107)和正常对照组(n=136);DM组根据有无并发肾病分为DN( )组(n=53)与DN(-)组(n=54)。分别用突变基因分离聚合酶链反应(MS-PCR)和PCR-限制性片段长度多态性(PCR-RFLP)技术对AGT M235T及AGT T174M多态性进行检测。结果]AGT基因M235T多态位点在DN( )、DN(-)及正常对照组中均以TT型多见,分别为0.717,0.667,0.640;三组间基因型分布构成比比较及等位基因频率构成比比较均无显著性差异。三组间AGT T174M基因型构成比比较及等位基因频率构成比比较均无统计学意义。结论]未发现AGT基因M235T或T174M多态性与广西地区2型DN的发生有关联。

Associations of AGT Gene Polymorphisms with Type 2 Diabetic Nephropathy in Guangxi People

Objective]To investigate whether or not AGT M235T and AGT T174M,two types of polymorphisms of AGT gene,are associated with type 2 diabetic nephropathy(DN) in Guangxi people.Methods]A case-control study was performed.243 subjects from Guangxi were classified into a type 2 diabetes mellitus(DM) group(n=107) and a control group(n=136).The former was subdivided into a DN( ) group(n=53) and a DN(-) group(n=54) according to with or without DN.The AGT M235 and AGT T174M polymorphisms were examed by mutagenically separated PCR(MS-PCR) and PCR-restriction fragment length polymorphism(PCR-RFLP),respectively.Results]The genotype found in AGT M235T polymorphic site was mostly TT type in three groups(0.717,0.667 and 0.640,respectively).No significant differences in AGT M235T genotypes and allelic frequencies were observed among three groups.The AGT T174M polymorphism did not display any difference in allelic frequencies and genotype distribution among three groups.Conclusions]AGT M235T and AGT T174M are not associated with the development of DN in Guangxi patients with type 2 DM.