母鼠孕期及哺乳期暴露双酚A对子代雌鼠体重及糖代谢的影响

目的研究孕期和哺乳期暴露双酚A对子代雌性大鼠体重及糖代谢的影响。方法 SD孕鼠从妊娠第6天至哺乳期结束饮用含1μg/ml双酚A的水,测量子代雌鼠从出生到断乳后5周末的体重变化情况,断乳后第5周空腹血糖、血清胰岛素和瘦素水平及主要脏器(肝、肾)和肾周脂肪、生殖器周围脂肪重量,并计算脏器系数及脂肪系数。结果与对照组比较,实验组子代雌鼠出生体重明显增加,增高的体重一直持续到实验结束。断乳后第5周,实验组子代雌鼠生殖器系数、生殖器周围脂肪系数明显增高,空腹血糖、胰岛素水平显著增高,血清瘦素水平明显降低,以上差异均有统计学意义(P<0.05或P<0.01)。结论发育早期暴露双酚A使雌鼠体重明显增高,并出现胰岛素抵抗现象;双酚A暴露引起的血清瘦素水平降低是肥胖和胰岛素抵抗发生的原因之一。

Effect of bisphenol A exposure during early development on body weight and glucose metabolism of female filial rats

Objective To investigate the effect of bisphenol A(BPA) exposure during early development on body weight and glucose metabolism of female filial rats.Methods Pregnant Sprague-Dawley rats were exposed by drinking water containing 1μg/ml BPA from the 6th day of gestation to the end of lactation.Body weight of female pups was measured on born,during lactation and after weaning.The levels of fasting blood glucose,insulin and leptin,the weight of liver and kidney and peri-gonadal and peri-renal adipose tissue were measured,and the organ and adipose tissue coefficients were calculated.Results Compared with the control pubs,the body weight of pups in BPA-exposed group was significantly higher at born and after weaning,the virginal and gonadal adipose tissue coefficient was significantly higher,and the levels of fasting blood glucose,serum insulin were increased but leptin decreased.Conclusion BPA exposure during early development could increase the body weight and result in insulin resistance.The decrease of serum leptin resulted from BPA exposure maybe one of reasons related to obesity and insulin resistance.