| 真菌性鼻窦炎5种真菌染色方法的比较 |
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| 引用本文： | 张勇,朴颖实,何春燕,刘红刚.真菌性鼻窦炎5种真菌染色方法的比较[J].诊断病理学杂志,2008,15(6). |
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| 作者姓名： | 张勇 朴颖实 何春燕 刘红刚 |
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| 作者单位： | 首都医科大学附属北京同仁医院病理科,北京,100730 |
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| 摘 要： | 目的对比分析不同的真菌染色方法在真菌性鼻窦炎真菌染色中的特点及其应用。方法91例真菌性鼻窦炎的石蜡包埋组织标本,真菌类型包括曲霉菌属真菌52例、链格孢菌2例、白色念珠菌12例和毛霉目真菌25例。连续切片,进行HE、PAS、AB-PAS、GMS和MUC5B免疫组化染色。结果曲霉菌属真菌和白色念珠菌PAS、AB-PAS、GMS和MUC5B免疫组化染色效果很好;链格孢菌GMS染色效果最佳,呈明显的棕黑色,其他染色方法真菌均呈现自身的棕色;毛霉目真菌除MUC5B外,其余染色方法均着色;当背景组织为丰富的黏液时,AB-PAS法的染色效果优于单纯的PAS染色,真菌和背景反差较大,真菌轮廓明显;所有类型真菌HE染色的效果均欠佳,真菌轮廓不够明显。结论真菌染色可联合应用PAS、AB-PAS、GMS和MUC5B免疫组化染色方法,其各有优势。其中GMS染色显示真菌效果最佳,所有真菌均着色;组织背景为丰富的黏液时,AB-PAS的染色效果优于单纯的PAS染色;MUC5B染色方法具有一定的特异性,能鉴别出形态接近的曲霉菌和毛霉菌。
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| 关 键 词： | 真菌 鼻窦炎 染色方法 |
Comparison among five staining methods of detecting fungi in fungal sinusitis |
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| ZHANG Yong,PIAO Ying-shi,HE Chun-yan,et al..Comparison among five staining methods of detecting fungi in fungal sinusitis[J].Chinese Journal of Diagnostic Pathology,2008,15(6). |
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| Authors: | ZHANG Yong PIAO Ying-shi HE Chun-yan |
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| Institution: | ZHANG Yong,PIAO Ying-shi,HE Chun-yan,et al.(Department of Pathology,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China) |
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| Abstract: | Objective To study the characteristics and application of different staining methods of detecting fungi in fungal sinusitis.Methods Paraffin-embedded blocks of 91 fungal sinusitis specimens were obtained,including 52 Aspergillus spp,2 Alternaria,12 C albicans and 25 Mucorales.We performed HE stain,PAS stain,AB-PAS stain,GMS stain,and MUC5B immunohistochemistry in all the cases.Results Aspergillus spp and C albicans were positive with all staining methods;the best method for detecting Alternaria was GMS stai... |
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| Keywords: | Fungus Sinusitis Staining method |
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