Effects of Sucrose Oleate and Sucrose Laureate on in Vivo Human Stratum Corneum Permeability |
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Authors: | Ayala-Bravo Hilda A Quintanar-Guerrero David Naik Aarti Kalia Yogeshvar N Cornejo-Bravo José M Ganem-Quintanar Adriana |
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Institution: | (1) División de Estudios de Posgrado (Tecnología Farmacéutica), Facultad de Estudios Superiores Cuautitlan/Universidad Nacional Autónoma de México, Av. 1° de Mayo s/n, Cuautitlán Izcalli, Estado de México, México, 54704;(2) Centre Interuniversitaire de Recherche et d'Enseignement, F-74160 Archamps, France;(3) Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Calzada Universidad, Mesa de Otay, 22300 Tijuana B.C., México |
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Abstract: | Purpose. The purpose of this work was to 1) investigate the effect of sucrose esters (sucrose oleate and sucrose laureate in water or in Transcutol®, TC) on the stratum corneum (SC) barrier properties in vivo and 2) examine the impact of these surfactant-like molecules on the in vivo percutaneous penetration of a model penetrant 4-hydroxybenzonitrile (4-HB).
Methods. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and transepidermal water loss measurements were used to evaluate the sucrose oleate- and sucrose laureate-induced biophysical changes in SC barrier function in vivo. In addition, the effect of the enhancers on 4-HB penetration was monitored in vivo using ATR-FTIR spectroscopy in conjunction with tape-stripping of the treated site.
Results. Treatment of the skin with 2% sucrose laureate or sucrose oleate in TC significantly increased the extent of 4-HB penetration relative to the control. Furthermore, when skin treated with these formulations was examined spectroscopically, the C-H asymmetric and symmetric stretching bands of the lipid methylene groups were characterized by 1) decreased absorbances and 2) frequency shifts to higher wavenumbers. These effects on the SC lipids and 4-HB penetration were more pronounced for sucrose laureate when combined with TC.
Conclusions. A combination of sucrose esters (oleate or laureate) and TC is able to temporally alter the stratum corneum barrier properties, thereby promoting 4-HB penetration. These molecules are worthy of further investigation as potential candidates for inclusion in transdermal formulations as penetration enhancers. |
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Keywords: | skin penetration penetration enhancement sucrose esters ATR-FTIR transepidermal water loss |
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