764—3对实验性炎症的保护作用

气管内一次滴注博莱霉素复制大鼠肺部炎症和胸膜腔内注入角叉菜胶复制胸膜炎动物模型。观察到中药丹参提取的有效单体,764-3对两种炎症模型均有保护作用。明显抑制博莱霉素引起的肺系数增加、肺组织及支气管肺泡灌洗液(BALF)中组胺含量的增加、BALF中β-g活性之升高及细胞计数之增多。对角叉菜胶引起的胸膜炎胸腔渗出液量及其中细胞计数、蛋白含量及β-g活性之增高也有明显的抑制作用。该抑制作用有剂量依赖关系。注射给药较口服给药作用稳定。

Protective effect of 764-3 on experimental inflammation

The antiinflammatory effect of 764-3 was investigated in rat models of lung inflammation and pleurisy induced by bleomycin intratracheal instillation and carrageenan intrapleural cavity injection respectively. 4 mg/100 g BW.d 764-3 hypodermal injection significantly inhibited the lung inflammatory changes in bleomycin treated rats. Compared with the experimental group, lung index, histamine content in lung tissue and in bronchoalveolar lavage fluid (BALF), number of PMN in BALF and BALF beta-glucuronidase activity significantly deceased in the therapeutic rats. 8 mg/100 g BW 764-3 intramuscular injection significantly inhibited the volume, number of PMN, protein content and beta-glucuronidase activity increase in carrageenan-induced pleural exudate fluid. The antiinflammatory effect of 8 mg/100 g BW 764-3 was equal to that of 3 mg/100 g BW hydrocortisone. 4 mg/100 g BW 764-3 injection significantly inhibited the increases in PMN numbers and beta-glucuronidase activity in pleural exudate fluid. 2 mg/100 g BW 764-3 injection significantly inhibited the PMN increase in pleural exudate fluid only. 1 mg/100 g BW 764-3 injection showed no antiinflammatory effect. When 764-3 was administered orally, the antiinflammatory effect was unsatisfactory. 5 mg/100 mg BW 764-3 significantly inhibited the volume, number of PMN, protein content and beta-glucuronidase activity of carrageenan-induced pleural exudate fluid. 2.5 mg/100 g BW 764-3 significantly inhibited the volume and protein content increases in pleural exudate fluid. But 3.5 mg/100 g BW 764-3 showed no antiinflammatory effect. The authors indicate that the antiinflammatory effect of orally administered 764-3 was not stable.