| 人宫颈鳞状上皮细胞癌克隆细胞系的研究：Ⅱ.HGPRT—突... |
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| 引用本文： | 王瑞瑜,田尔明.人宫颈鳞状上皮细胞癌克隆细胞系的研究：Ⅱ.HGPRT—突...[J].中国医学科学院学报,1992,14(6):461-464. |
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| 作者姓名： | 王瑞瑜 田尔明 |
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| 摘 要： |
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| 关 键 词： | 子宫颈肿瘤 突变型 克隆 细胞 |
Studies on human cervical carcinoma cell line. II. Establishment of HGPRT- cell line CC-80IAR2 and its biological characteristics] |
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| R Wang.Studies on human cervical carcinoma cell line. II. Establishment of HGPRT- cell line CC-80IAR2 and its biological characteristics][J].Acta Academiae Medicinae Sinicae,1992,14(6):461-464. |
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| Authors: | R Wang |
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| Institution: | Institute of Basic Medical Sciences, Beijing. |
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| Abstract: | An HGPRT- cell line, CC-801AR2, was established from cloned human cervical carcinoma cell line CC-801 by using MNNG to induce cell mutation and 8-azaguanine (8-AG) to select the 8-AG resistant cells. The deficiency of HG-PRT in CC-801AR2 cells was proved by enzyme activity assay. This cell line was very sensitive to HAT medium. It was very similar in biological characteristics to CC-801, especially in that it retained its tumorigenicity when transplanted into nude mice. CC-801AR2 can therefore be used in somatic cell hybridization and gene transfer experiments. |
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