| HPLC法测定麸炒山药饮片中尿囊素的含量 |
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| 作者姓名： | 张芳芳 李伟东 杨光明 曾庆琪 蔡宝昌 刘雪东 |
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| 作者单位： | 1. 南京中医药大学江苏省中药炮制重点实验室,江苏,南京,210029
2. 江苏建康职业学院,江苏,南京,210029 |
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| 基金项目： | 国家药典委员会(YZ-22) |
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| 摘 要： | 目的建立麸炒山药饮片中尿囊素含量测定的方法,测定不同产地麸炒山药饮片中尿囊素的含量。方法采用ODS-C18柱(250 mm×4.6 mm,5μm);流动相:甲醇-磷酸二氢钾水溶液(2∶98);流速0.8 mL/min;柱温:30℃;检测波长:224 nm。结果尿囊素在0.144~2.312μg范围内线性关系良好,r=0.9999;尿囊素的平均回收率为99.05%,RSD=2.99%(n=9)。结论该方法可为麸炒山药饮片的质量控制提供依据,不同产地麸炒山药中尿囊素含量差异较大。
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| 关 键 词： | 尿囊素 麸炒山药 HPLC 含量测定 |
Detection of Allantoin in Bran-baked Sliced Rhizoma Dioscoreae with HPLC |
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| Authors: | ZHANG Fang-fang LI Wei-dong YANG Guang-ming ZENG Qing-qi CAI Bao-chang LIU Xue-dong |
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| Institution: | 1Nanjing University of Chinese Medicine/a>;Nanjing/a>;210029/a>;China/a>;2Jiangsu Key Laboratory of Chinese Medicine Processing/a>;3Jiangsu Jiangkang College staff Medical/a>;China |
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| Abstract: | OBJECTIVE To establish a method for the determination of allantoin and determine content of allantoin in Rhizoma Dioscoreae Preparata from different producing areas.METHODS Separation and determination of allantoin were accessed by using an ODS-C18 column and a mobile phase8 of methanol - potassium dihydrogen phosphate aqueous solution at a ratio of 2:98 (v/v). The flow rate was 0.8 mL/min, temperature at 30℃ and detection wavelength at 224 nm. RESULTS A good linearity of allantoin was achieved in the range of 0.144 - 2.132 μg, r= 0.9999; the mean value of recovery is 99.05%, RSD is 2.99%. CONCLUSIONS The method in this paper could provide an evidence for quality control of Rhizoma Dioscoreae Preparata and there is comparatively large difference of content of allantoin in Rhizoma Dioscoreae Preparata from different producing areas. |
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| Keywords: | allantoin Rhizoma Dioscoreae Preparata HPLC content determination |
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