| MSP法检测膀胱移行细胞癌PTEN启动子甲基化的实验研究 |
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| 引用本文： | 刘燕,孙华宾,延敏博,徐白生. MSP法检测膀胱移行细胞癌PTEN启动子甲基化的实验研究[J]. 中国卫生检验杂志, 2012, 0(6): 1305-1306 |
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| 作者姓名： | 刘燕 孙华宾 延敏博 徐白生 |
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| 作者单位： | 中山大学附属第五医院 |
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| 基金项目： | 广东省医学科研基金(B2011368);广东省科技计划项目(2010B06090042) |
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| 摘 要： | 目的:探讨甲基化特异性PCR(MSP)法检测膀胱移行细胞癌PTEN基因启动子甲基化的方法及意义。方法:应用甲基化特异性PCR(MSP)法检测膀胱移行细胞癌组织(48例)和正常膀胱组织(15例)PTEN基因启动子甲基化状态。结果:膀胱癌组织中PTEN启动子甲基化率为47.9%(23/48),而在15例正常膀胱组织中其启动子未发生甲基化,两组间差异具有统计学意义(P<0.01)。结论:MSP法检测膀胱移行细胞癌PTEN基因启动子甲基化可行。
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| 关 键 词： | 膀胱移行细胞癌 PTEN 甲基化 |
Study on promoter methylation of PTEN gene in transitional cell carcinoma of the bladder by MSP |
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| LIU Yan,SUN Hua-bin,YAN Min-bo,XU Bai-sheng. Study on promoter methylation of PTEN gene in transitional cell carcinoma of the bladder by MSP[J]. Chinses Journal of Health Laboratory Technology, 2012, 0(6): 1305-1306 |
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| Authors: | LIU Yan SUN Hua-bin YAN Min-bo XU Bai-sheng |
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| Affiliation: | (The Fifth Affiliated Hospital of SUN Yat-Sen University,Zhuhai 519000,China) |
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| Abstract: | Objective:To explore the method and significance of promoter methylation of PTEN gene in transitional cell carcinoma of the bladder(TCCB).Methods:A total of 48 patients with TCCB and 15 normal controls were enrolled into this study.Methylation specific PCR(MSP) techniques were used to detect the PTEN gene promoter methylation expression status in these two groups.Results:The rate of methylation was 47.9%(23/48) in TCCB cases,while none was found in normal controls.There was statistical significance between the two groups.(P<0.01).Conclusion:MSP techniques were feasible in detection of PTEN gene promoter methylation in transitional cell carcinoma of the blaader(TCCB). |
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| Keywords: | Transitional cell carcinoma of bladder PTEN Methylation |
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