| HPLC-ELSD法测定穿山龙中薯蓣皂苷元的含量 |
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| 引用本文： | 李艳荣,李强,洪霞,潘海峰.HPLC-ELSD法测定穿山龙中薯蓣皂苷元的含量[J].承德医学院学报,2011,28(2):126-128. |
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| 作者姓名： | 李艳荣 李强 洪霞 潘海峰 |
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| 作者单位： | 李艳荣,洪霞,潘海峰(承德医学院中药研究所,河北承德,067000);李强(宽城县医院) |
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| 摘 要： | 目的:建立高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定穿山龙中薯蓣皂苷元含量的方法.方法:使用Agilent Eclipse C18(150×4.6mm,5μm)色谱柱,流动相为甲醇-水(95:5),柱温:40℃,流速1.0ml/min;蒸发光检测器以N2为载气,载气压力3.5bar,漂移管温度为70℃.结...
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| 关 键 词： | 穿山龙 薯蓣皂苷元 高效液相色谱法 蒸发光检测器 |
DETERMINATION OF DIOSGENIN CONTENT IN DIOSCOREA NIPPONICA MAKINO BY HPLC-ELSD |
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| LI Yan-rong,LI Qiang,HONG Xia,et al.DETERMINATION OF DIOSGENIN CONTENT IN DIOSCOREA NIPPONICA MAKINO BY HPLC-ELSD[J].Journal of Chengde Medical College,2011,28(2):126-128. |
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| Authors: | LI Yan-rong LI Qiang HONG Xia |
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| Institution: | LI Yan-rong,LI Qiang,HONG Xia,et al(Institude of Chinese Materia,Chengde Medical College,Hebei Chengde,067000) |
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| Abstract: | Objective:To establish a testing method for determination of diosgenin content in Dioscorea nipponica Makino by PHLC-ELSD.Methods:Agilent Eclipse C18(150mm×4.6mm,5μm)column and evaporated light scattering detector(N2 as carrier gas,pressure:3.5bar,drift tube temperature:70℃) were used in this study.Mobile phase was methanol-water(95:5) at 40℃,flow rate was 1.0ml/min.Results:Diosgenin had better linear rate at 2.04-0.255mg/ml(r=0.9996),the average recovery was 98.9%(RSD=1.4%);and this PHLC-ELSD method had better repeatability.Conclusions:This method for detecting diosgenin content in Dioscorea nipponica Makino by PHLC-ELSD is specific,sensitive and accurate,can be acted as effective method for quality control of Dioscorea nipponica Makino. |
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| Keywords: | Dioscorea nipponica Makino Diosgenin HPLC Evaporated light scattering detector |
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