| 烟草Ⅰ类几丁质酶和β-1,3葡聚糖酶基因茎尖转化太子参的研究 |
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| 引用本文： | 王贵,刘敬梅,高山林. 烟草Ⅰ类几丁质酶和β-1,3葡聚糖酶基因茎尖转化太子参的研究[J]. 药物生物技术, 2006, 13(5): 334-337 |
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| 作者姓名： | 王贵 刘敬梅 高山林 |
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| 作者单位： | 1. 中国药科大学遗传育种教研室,江苏,南京,210038
2. 北京扬华生物科技有限公司,北京,102209 |
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| 摘 要： | 以烟草为材料，对经过修饰的烟草Ⅰ类几丁质酶和Ⅰ类β-1，3-葡聚糖酶cDNA进行了克隆和序列分析，然后构建到一个双-T植物表达载体130上。用太子参无菌苗的茎尖分生组织为受体，采用农杆菌介导法进行转化。感染小苗经共培养后用除草剂筛选。然后对除草剂抗性植株进行分子检测，获得了转基因植株。
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| 关 键 词： | 茎尖 转化 太子参 葡聚糖酶基因 几丁质酶基因 |
| 文章编号： | 1005-8915（2006）05-0334-04 |
| 收稿时间： | 2005-01-22 |
| 修稿时间： | 2005-01-222006-04-27 |
Introduction of Tobacco Ⅰ-Chitinase and Ⅰ-β-1, 3-Glucanase Gene into pseudostellaria heterophylla following Transformation with Shoot Apex |
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| WANG Gui-rong,LIU Jing-mei,GAO Shan-lin. Introduction of Tobacco Ⅰ-Chitinase and Ⅰ-β-1, 3-Glucanase Gene into pseudostellaria heterophylla following Transformation with Shoot Apex[J]. Pharmaceutical Biotechnology, 2006, 13(5): 334-337 |
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| Authors: | WANG Gui-rong LIU Jing-mei GAO Shan-lin |
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| Affiliation: | 1. Department of Genetics and Breeding, China Pharmaceutical Uiversity, Nanjing 210038, China; 2. Beijing yanghua biotechnology co. 1td, Beijing 102209, China |
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| Abstract: | The modified I-chitinase and I-1,3-glucanase from the Nicotiana tabacumcv NC89 were cloned and sequenced.Then they were cloned into a plant double-T expression vector.The apical meristems of Pseudostellaria heterophylla plantlets coming from sterilized seeds were used as the receptor of Agrobacterium-mediated transformation.After cocultivation,the plantlets were transferred to selected media.Fertile transgenic plants were obtained from herbicide-resistant ones and confirmed by PCR and PCR-southern assay. |
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| Keywords: | Shoot apext Transformation Pseudostellaria heterophylla Chitinase gene |
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