重组人生长激素在治疗肝硬变大鼠肝部分切除术后低白蛋白血症中的作用

目的探讨重组人生长激素(rhGH)在治疗肝硬变大鼠肝部分切除术后低白蛋白血症中的作用。方法30只大鼠按编号法随机分为正常对照组、肝硬变组(LC组)、肝硬变切肝组(LCH组)、PN组(于肝叶切除术后行PN治疗)及rhGH PN组(于肝叶切除术后行rhGH PN治疗)．每组6只。检测大鼠肝功能、血糖．用RT-PCR法检测肝组织ALB mRNA的表达．肝组织行Ki67免疫组化染色。结果与PN组比较．rhGH PN组血清ALP降低，ALB、血糖升高．肝组织ALBmRNA表达水平及肝组织Ki67指数均升高。结论重组人生长激素可以改善肝硬变大鼠肝部分切除后低白蛋白血症。

Effects of Recombinant Human Growth Hormone on Hypoalbuminemia in Cirrhotic Rats after Partial Hepatectomy

Objective To investigate the effects of rhGH on hypoalbuminemia in cirrhotic rats after partial hepatectomy. Methods Thirty rats were randomly divided into normal control group ( n =6), liver cirrhosis group (LC group, n =6), liver cirrhosis and hepatectomy group (LCH group, n =6), PN (parenteral nutrition) group ( n =6, given PN after hepatectomy) and rhGH+PN group ( n =6,given rhGH and PN after hepatectomy). Liver function and blood glucose were measured. The expression of ALB mRNA was detected by RT PCR. Liver Ki67 immunohistochemistry was studied. Results Compared with PN group, serum ALP was lower; serum ALB and blood glucose were higher in rhGH+PN group. The expression of hepatic ALB mRNA was higher, and hepatic Ki67 labeling index was higher as well in this group. Conclusion rhGH can improve hypoalbuminemia after partial hepatectomy in cirrhotic rats with partial hepatectomy.