The swine CD81 enhances E2-based DNA vaccination against classical swine fever |
| |
| Affiliation: | 1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture; National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China;2. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;1. Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan;2. Department of Pathology, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan;3. Division of Experimental Animals Research, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo 208-0011, Japan;4. Department of Clinical Laboratory, Affiliated Hospital of Qingdao University Medical College, Jiangsu Road 16, Qingdao 266003, China;5. Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0781, Japan;1. Clinic for Swine at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians University, Sonnenstrasse 16, 85764 Oberschleissheim, Germany;2. Institute for Infectious Diseases and Zoonosis, Ludwig-Maximilians University, 80539 Munich, Germany;3. Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians University, 80539 Munich, Germany;4. Zoetis GmbH, Schellingstraße 1, 10785 Berlin, Germany;1. Section for Virology, DTU National Veterinary Institute, Technical University of Denmark, Lindholm, DK-4771 Kalvehave, Denmark;2. Section for Molecular Disease Biology, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlaegevej 88, 1, DK-1870 Frederiksberg C, Denmark;1. Department of Veterinary Science, University of Parma, Strada del Taglio, 10 – 43126, Parma, Italy;2. Department of Medicine and Surgery, University of Parma, Via Gramsci, 14 – 43126, Parma, Italy;1. Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald-Insel Riems, Germany;2. Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institut, Suedufer 10, 17493 Greifswald-Insel Riems, Germany |
| |
| Abstract: | Classical swine fever (CSF) is a highly contagious and economically important viral disease that affects the pig industry worldwide. The glycoprotein E2 of CSFV can induce neutralizing antibodies and protective immunity, and is widely used for novel vaccine development. The objective of this study was to explore whether a tetraspanin molecule CD81 could improve the immune responses of an E2-based DNA vaccine. Plasmids pVAX-CD81, pVAX-E2 and pVAX-CD81-E2 were constructed and the expression of target proteins was confirmed in BHK-21 cells by indirect immunofluorescence assay. BALB/c mice were divided into 5 groups and immunized with different plasmids (pVAX-E2, pVAX-CD81-E2, pVAX-E2 + pVAX-CD81, pVAX-CD81 and PBS) three times with two weeks interval. The results showed that the introduction of CD81 promoted higher humoral and cellular immune responses than E2 expression alone (P < 0.05). In addition, immunization with pVAX-CD81-E2 induced stronger immune responses than pVAX-E2 + pVAX-CD81. Furthermore, four groups of pigs were immunized with pVAX-E2, pVAX-CD81-E2, pVAX-CD81 and PBS, respectively. Humoral and cellular immune responses detection showed similar results with those in mice. Compared to pVAX-E2, pVAX-CD81-E2 induced higher titers of neutralizing antibodies after viral challenge and conferred stronger protection. These results confirmed the capacity of swine CD81 enhancing the humoral and cellular responses with an adjuvant effect on CSFV DNA vaccine. This is the first report demonstrating the adjuvant effect of CD81 to enhance the DNA vaccination for swine pathogen. |
| |
| Keywords: | CSFV DNA vaccine CD81 E2 glycoprotein Adjuvant |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
