An influenza viral vector Brucella abortus vaccine induces good cross-protection against Brucella melitensis infection in pregnant heifers |
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| Institution: | 1. Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires, Argentina;2. Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, UBA, Buenos Aires, Argentina;3. Instituto de Inmunología, Genética y Metabolismo (INIGEM), Laboratorio de Inmunogenética, Hospital de Clínicas “José de San Martín”, CONICET-UBA, Buenos Aires, Argentina;1. Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL, USA;2. Howard Taylor Ricketts Laboratory, The University of Chicago, Argonne National Laboratory, Argonne, IL, USA;3. Department of Pathology, The University of Chicago, Chicago, IL, USA;4. Department of Microbiology, The University of Chicago, Chicago, IL, USA;1. Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box: 14115-175, Tehran, Iran;2. Department of Genetics, Science and Biotechnology Research Center, Mallek-Ashtar University of Technology, P.O. Box: 15875-1774, Tehran, Iran;3. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box: 14115-111, Tehran, Iran;1. Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran;2. Department of Molecular Immunology and Vaccine Research, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran;3. Department of Bacteriology, Pasteur Institute of Iran, Pasteur Street, Tehran, Iran;4. Department of Bacterial Vaccine and Antigen Production, Pasteur Institute of Iran, Karaj, Iran;1. Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, State Key Laboratory for Agrobiotechnolgy, National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China;2. China Institute of Veterinary Drug Control, Beijing, China;3. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, Shandong, China |
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| Abstract: | Brucella melitensis can be transmitted and cause disease in cattle herds as a result of inadequate management of mixed livestock farms. Ideally, vaccines against Brucella abortus for cattle should also provide cross-protection against B. melitensis. Previously we created a novel influenza viral vector B. abortus (Flu-BA) vaccine expressing the Brucella ribosomal proteins L7/L12 or Omp16. This study demonstrated Flu-BA vaccine with adjuvant Montanide Gel01 provided 100% protection against abortion in vaccinated pregnant heifers and good cross-protection of the heifers and their calves or fetuses (90–100%) after challenge with B. melitensis 16 M; the level of protection provided by Flu-BA was comparable to the commercial vaccine B. abortus S19. In terms of the index of infection and colonization of Brucella in tissues, both vaccines demonstrated significant (P = 0.02 to P < 0.0001) protection against B. melitensis 16 M infection compared to the negative control group (PBS + Montanide Gel01). Thus, we conclude the Flu-BA vaccine provides cross-protection against B. melitensis infection in pregnant heifers. |
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| Keywords: | Influenza viral vector Vaccine Cross-protection Pregnant heifer |
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